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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Preparatory production of quercetin-3-β-d-glucopyranoside using alkali-tolerant thermostable α-l-rhamnosidase from Aspergillus terreus
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Preparatory production of quercetin-3-β-d-glucopyranoside using alkali-tolerant thermostable α-l-rhamnosidase from Aspergillus terreus

机译：使用耐碱的热稳定α-1-鼠李糖苷酶从曲霉中制备槲皮素-3-β-d-吡喃葡萄糖苷

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摘要

Extensive screening for a robust producer of α-. l-rhamnosidase activity from well-defined strains of filamentous fungi, including multifactorial optimization (inducers, cultivation conditions) was accomplished. Enzyme production of the optimal producer. Aspergillus terreus (non-toxigenic) was scaled up to 50. L. α-. l-Rhamnosidase, which was fully characterized, proved to be thermo- and alkali-tolerant, thus enabling effective operation at 70. °C and pH 8.0. These conditions allow for a very high substrate (rutin) load up to 100-300. g/L, thus enabling very high volumetric productivity of the reaction product quercetin-3-β-. d-glucopyranoside (isoquercitrin). Here, a novel concept of " immobilised substrate" is used. Isoquercitrin is a highly effective and biocompatible antioxidant with strong anti-inflammatory activities. Rutin biotransformation was optimized and scaled up to ca 10. kg production and thus the robustness of the large-scale production was demonstrated. Isoquercitrin can be produced to a very high purity (98%) in multikilogram amounts, without any quercetin and directly applicable in nutraceuticals.
机译：广泛筛选α-的可靠产生者。实现了来自明确定义的丝状真菌菌株的l-鼠李糖苷酶活性，包括多因素优化（诱导剂，培养条件）。酶生产的最佳生产者。曲霉曲霉（无毒）可放大至50. L.α-。 l-鼠李糖苷酶具有充分的特性，被证明具有耐热性和耐碱性，因此能够在70°C和pH 8.0下有效运行。这些条件允许很高的基材（芦丁）负载，最高可达100-300。 g / L，因此使得反应产物槲皮素-3-β-的体积生产率非常高。 d-吡喃葡萄糖苷（异槲皮苷）。在此，使用“固定化基材”的新概念。异槲皮苷是一种高效且具有生物相容性的抗氧化剂，具有强大的抗炎活性。对芦丁的生物转化进行了优化，并将其规模扩大到约10公斤/千克，从而证明了大规模生产的稳健性。异槲皮苷可以以几千克的量生产出非常高的纯度（98％），而无需任何槲皮素，可直接用于营养保健品。

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