• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and experimental allergy : >Dendritic cell and T cell responses in children with food allergy.
【24h】

Dendritic cell and T cell responses in children with food allergy.

机译：食物过敏儿童的树突状细胞和T细胞反应。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Food allergy (FA) and eosinophilic oesophagitis (EE) are increasingly common clinical problems. Dendritic cells (DCs) are key regulators of the sensitization and effector phases of allergic immune responses, but their role in these diseases is largely unknown. OBJECTIVE: To evaluate for alterations in the phenotype and function of DCs in children with IgE-mediated milk allergy or EE compared with their non-affected siblings. METHODS: Plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) were prepared from peripheral blood of children with milk allergy (FA), EE, and non-affected siblings (CON). Purified pDCs and mDCs were cultured alone or with autologous CD4(+) lymphocytes. Cytokine levels in plasma, or culture supernatants following stimulation, were measured using multiplex array immunoassay. Cell-surface molecule expression was determined by flow cytometry. RESULTS: DCs from FA subjects produced greater levels of pro-inflammatory cytokines (IL-6, TNF-alpha), granulocyte macrophage-colony forming factor, and mDC-derived IL-10 compared with controls following allergen exposure. T(H) 2 but not T(H) 1 cytokines were spontaneously produced in DC-CD4(+) T cell co-cultures from children with FA and were not significantly increased after stimulation with milk extract, suggesting an ongoing activation in vivo. This hypothesis was further supported by evidence for elevated IL-5 and IL-13 protein in the plasma of children with both FA and EE. The only significant DC phenotypic differences were: (1) reduced levels of CD80 in EE subjects and (2) FcvarepsilonRI expression that correlated with serum IgE levels in both groups of subjects. CONCLUSION: This study suggests that DCs from children with FA and EE produce more pro-inflammatory cytokines, and that their CD4(+) T cells are spontaneously activated to produce T(H) 2 cytokines in the presence of FcvarepsilonRI-bearing DCs.
机译：背景：食物过敏（FA）和嗜酸性食管炎（EE）是越来越普遍的临床问题。树突状细胞（DC）是过敏性免疫反应的敏化和效应期的关键调节因子，但在这些疾病中的作用很大程度上未知。目的：评估与未患病的兄弟姐妹相比，IgE介导的牛奶过敏或EE儿童患儿DC的表型和功能改变。方法：从患有牛奶过敏（FA），EE和未患病兄弟姐妹（CON）的儿童的外周血中制备浆细胞样DC（pDC）和髓样DC（mDC）。纯化的pDC和mDC可以单独培养或与自体CD4（+）淋巴细胞一起培养。使用多重阵列免疫测定法测量血浆或刺激后培养上清液中的细胞因子水平。细胞表面分子表达通过流式细胞术确定。结果：与暴露于过敏原的对照组相比，来自FA受试者的DC产生更高水平的促炎细胞因子（IL-6，TNF-α），粒细胞巨噬细胞集落形成因子和mDC衍生的IL-10。 T（H）2但不是T（H）1细胞因子是由患有FA的儿童在DC-CD4（+）T细胞共培养物中自发产生的，并且在用牛奶提取物刺激后并没有显着增加，表明体内正在进行的活化。 FA和EE患儿血浆中IL-5和IL-13蛋白升高的证据进一步支持了这一假设。唯一显着的DC表型差异是：（1）EE受试者中CD80水平降低，以及（2）两组受试者中与血清IgE水平相关的FcvarepsilonRI表达。结论：这项研究表明，患有FA和EE的儿童的DC产生更多的促炎细胞因子，并且在带有携带FcvarepsilonRI的DC的情况下，它们的CD4（+）T细胞被自然激活以产生T（H）2细胞因子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号