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首页> 外文期刊>Clinical and experimental allergy : >Role of fungal antigens in eosinophilia-associated cellular responses in nasal polyps: a comparison with enterotoxin.
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Role of fungal antigens in eosinophilia-associated cellular responses in nasal polyps: a comparison with enterotoxin.

机译：鼻息肉中真菌抗原在嗜酸性粒细胞相关细胞反应中的作用：与肠毒素的比较。

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BACKGROUND: Fungi and/or Staphylococcus aureus enterotoxins (SEs) may participate in the pathogenesis of eosinophilic inflammation in cases of chronic rhinosinusitis with nasal polyps (CRSwNP). Objective We sought to determine the effects of fungal antigens on eosinophilia-associated cellular responses in nasal polyps. METHODS: Dispersed nasal polyp cells (DNPCs) were prepared from 13 patients with CRSwNP. DNPCs were cultured with fungal extracts (Aspergillus, Alternaria and Candida) or SEB for 72 h, after which the levels of IL-5, IL-13 and RANTES were measured within the supernatant. Responses to beta-d-glucan, mannan and chitin were also examined. RESULTS: 38.5%, 69.2% and 30.8% of DNPCs produced IL-5, IL-13 and RANTES, respectively, in response to 200 mug/mL of Aspergillus. 53.8%, 53.8% and 7.7% of DNPCs produced IL-5, IL-13 and RANTES, respectively, in response to 200 mug/mL of Alternaria. 53.8%, 38.5% and 15.4% of DNPCs produced IL-5, IL-13 and RANTES, respectively, in response to 200 mug/mL of Candida. All DNPCs produced these cytokines in response to 0.1 mug/mL of SEB. SEB induced significantly greater cytokine levels than the fungal extracts. No correlation between cytokine production following exposure to each of the fungal extracts or SEB and various clinical features, including nasal polyp eosinophilia and radiological severity of sinusitis was observed. Neither sensitization to fungus nor comorbidity with bronchial asthma was correlated with the fungal extract-induced cytokine production by DNPCs. beta-d-glucan, mannan and chitin did not induce significant cytokine production. CONCLUSIONS: These results suggest that, although DNPCs produce IL-5, IL-13 and RANTES in response to fungal extracts, fungal antigens including major carbohydrates are less capable of inducing eosinophilia-associated cellular responses in nasal polyps than SEB.
机译：背景：真菌性和/或金黄色葡萄球菌肠毒素（SEs）可能参与患有鼻息肉（CRSwNP）的慢性鼻-鼻窦炎的嗜酸性炎症反应。目的我们试图确定真菌抗原对鼻息肉中嗜酸性粒细胞相关细胞反应的影响。方法：从13例CRSwNP患者中制备鼻息肉分散细胞（DNPC）。将DNPC与真菌提取物（曲霉，链霉菌和念珠菌）或SEB一起培养72小时，然后在上清液中测量IL-5，IL-13和RANTES的水平。还检查了对β-d-葡聚糖，甘露聚糖和几丁质的反应。结果：响应200杯/毫升的曲霉菌，分别有38.5％，69.2％和30.8％的DNPC产生IL-5，IL-13和RANTES。响应200杯/毫升的链格孢菌，分别有53.8％，53.8％和7.7％的DNPC产生IL-5，IL-13和RANTES。响应200杯/ mL念珠菌，分别有53.8％，38.5％和15.4％的DNPC产生IL-5，IL-13和RANTES。所有DNPC都对0.1杯/毫升SEB产生这些细胞因子。 SEB诱导的细胞因子水平明显高于真菌提取物。暴露于每种真菌提取物或SEB后的细胞因子产生与各种临床特征（包括鼻息肉嗜酸性粒细胞增多和鼻窦炎的放射学严重程度）之间没有相关性。 DNPC对真菌致敏或与支气管哮喘合并症均与真菌提取物诱导的细胞因子产生无关。 β-d-葡聚糖，甘露聚糖和几丁质没有诱导明显的细胞因子产生。结论：这些结果表明，尽管DNPC对真菌提取物产生反应，产生IL-5，IL-13和RANTES，但与主要SEB相比，包括主要碳水化合物在内的真菌抗原在鼻息肉中诱导嗜酸性粒细胞相关细胞反应的能力较弱。

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