Biochemical Characterization of the O-Linked Glycosylation Pathway in Neisseria gonorrhoeae Responsible for Biosynthesis of Protein Glycans Containing N,N '-Diacetylbacillosamine

Hartley MD; Morrison MJ; Aas FE; Borud B; Koomey M; Imperiali B

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Biochemical Characterization of the O-Linked Glycosylation Pathway in Neisseria gonorrhoeae Responsible for Biosynthesis of Protein Glycans Containing N,N '-Diacetylbacillosamine

机译：淋病奈瑟氏球菌中O联糖基化途径的生物化学特性，负责生物合成包含N，N'-二乙酰基芽孢杆菌胺的蛋白质聚糖

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The O-linked protein glycosylation pathway in Neisseria gonorrhoeae is responsible for the synthesis of a complex oligosaccharide on undecaprenyl diphosphate and subsequent en bloc transfer of the glycan to serine residues of select periplasmic proteins. Protein glycosylation (pgl) genes have been annotated on the basis of bioinformatics and top-down mass spectrometry analysis of protein modifications in pgl-null strains [Aas, F. E., et al. (2007) Mol. Microbiol. 65, 607-624; Vik, A., et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 4447-4452], but relatively little biochemical analysis has been performed to date. In this report, we present the expression, purification, and functional characterization of seven Pgl enzymes. Specifically, the enzymes studied

机译：淋病奈瑟氏球菌中的O联蛋白糖基化途径负责在十一碳二烯基二磷酸上合成复杂的寡糖，随后将聚糖整体转移到所选周质蛋白的丝氨酸残基上。蛋白质糖基化（pgl）基因已根据生物信息学和pgl无效菌株中蛋白质修饰的自上而下质谱分析进行了注释[Aas，F. E.，et al。（2007）Mol。微生物。 65，607-624; Vik，A。等。（2009年）Proc。 Natl。学院科学U.S.A. 106，4447-4452]，但是迄今为止，已经进行了相对较少的生化分析。在此报告中，我们介绍了七个Pgl酶的表达，纯化和功能表征。具体来说，研究的酶

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《Biochemistry》 |2011年第22期|共13页
作者
Hartley MD; Morrison MJ; Aas FE; Borud B; Koomey M; Imperiali B;
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正文语种 eng
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IV PILUS STRUCTURE; CAMPYLOBACTER-JEJUNI; PILIN GLYCOSYLATION; FUNCTIONAL-CHARACTERIZATION; OLIGOSACCHARYL TRANSFERASE; GENETIC-CHARACTERIZATION; CHEMOENZYMATIC SYNTHESIS; FLAGELLIN GLYCOSYLATION; ESCHERICHIA-COLI; PHASE VARIATION;

机译：静脉曲张结构;蝶形虫-JEJUNI;比林糖基化;功能特性;寡糖基转移酶;遗传特性;化学酶法合成;氟菌素糖基化;大肠埃希氏菌-磷脂;相变;

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1. Biochemical Characterization of the O-Linked Glycosylation Pathway in Neisseria gonorrhoeae Responsible for Biosynthesis of Protein Glycans Containing N,N′-Diacetylbacillosamine [J] . Meredith D. Hartley Michael J. Morrison Finn Erik Aas Bente Børud Michael Koomey and Barbara Imperiali Biochemistry . 2011,第22期

机译：淋病奈瑟氏球菌中O联糖基化途径的生化特性，负责生物合成包含N，N'-二乙酰基芽孢胺的蛋白聚糖
2. Biochemical Characterization of the O-Linked Glycosylation Pathway in Neisseria gonorrhoeae Responsible for Biosynthesis of Protein Glycans Containing N,N '-Diacetylbacillosamine [J] . Hartley MD, Morrison MJ, Aas FE, Biochemistry . 2011,第22期

机译：淋病奈瑟氏球菌中O联糖基化途径的生物化学特性，负责生物合成包含N，N'-二乙酰基芽孢杆菌胺的蛋白质聚糖
3. Neisseria gonorrhoeae O-linked pilin glycosylation: functional analyses define both the biosynthetic pathway and glycan structure. [J] . Aas FE, Vik A, Vedde J, Molecular Microbiology . 2007,第3期

机译：淋病奈瑟氏球菌O-连接的菌毛蛋白糖基化：功能分析定义了生物合成途径和聚糖结构。
4. Profiling and Characterization of N- and O-linked Glycans Released from Glycoproteins using RP-HPLC with Charged Aerosol Detection and Mass Spectrometry [C] . Andrew J. S. Hanneman, Jason C. Rouse American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：使用RP-HPLC具有带电气溶胶检测和质谱法从糖蛋白释放的N-和O型链接聚糖的分析和表征
5. Characterization of the gene encoding Omp85, a novel outer membrane protein of Neisseria gonorrhoeae and introduction of genes into Neisseria gonorrhoeae. [D] . Reschke, Dennis Karl. 1998

机译：编码淋病奈瑟氏球菌的新型外膜蛋白Omp85的基因的鉴定，并将基因导入淋病奈瑟氏球菌。
6. Biochemical characterization of the O-linked glycosylation pathway in Neisseria gonorrhoeae responsible for biosynthesis of protein glycans containing NN’-diacetylbacillosamine [O] . Meredith D. Hartley, Michael J. Morrison, Finn Erik Aas, -1

机译：在奈瑟氏球菌的O-连接的糖基化途径的生物化学表征淋球菌负责含N蛋白聚糖生物合成N- diacetylbacillosamine
7. Biochemical Characterization of the O-Linked Glycosylation Pathway in Neisseria gonorrhoeae Responsible for Biosynthesis of Protein Glycans Containing N,N '-Diacetylbacillosamine [O] . Hartley, Meredith D., Morrison, Michael James, Imperiali, Barbara, 2011

机译：淋病奈瑟菌中O-连接糖基化途径的生化特征负责含有N，N'-二乙酰基青霉胺的蛋白质聚糖的生物合成

Biochemical Characterization of the O-Linked Glycosylation Pathway in Neisseria gonorrhoeae Responsible for Biosynthesis of Protein Glycans Containing N,N '-Diacetylbacillosamine

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