...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Isoenergetic Microarrays To Study the Structure and Interactions of DsrA and OxyS RNAs in Two- and Three-Component Complexes
【24h】

Isoenergetic Microarrays To Study the Structure and Interactions of DsrA and OxyS RNAs in Two- and Three-Component Complexes

机译：等能量微阵列研究二组分和三组分复合物中DsrA和OxyS RNA的结构和相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Information on the secondary structure and interactions of RNA is important to understand the biological function of RNA as well as in applying RNA as a tool for therapeutic purposes. Recently, the isoenergetic microarray mapping method was developed to improve the prediction of RNA secondary structure. Herein, for the first time, isoenergetic microarrays were used to study the binding of RNA to protein or other RNAs as well as the interactions of two different RNAs and protein in a three-component complex. The RNAs used as models were the regulatory DsrA and OxyS RNAs from Escherichia coli, the fragments of their target mRNAs (fhlA and rpoS), and their complexes with Hfq protein. The collected results showed the advantages and some limitations of microarray mapping.
机译：有关RNA二级结构和相互作用的信息对于理解RNA的生物学功能以及将RNA用作治疗目的的工具非常重要。近来，开发了等能微阵列作图法以改善RNA二级结构的预测。本文中，首次使用等能微阵列研究RNA与蛋白质或其他RNA的结合以及三组分复合物中两种不同RNA和蛋白质的相互作用。用作模型的RNA是来自大肠杆菌的调节DsrA和OxyS RNA，它们的靶mRNA（fhlA和rpoS）片段以及它们与Hfq蛋白的复合物。收集的结果表明了微阵列作图的优点和局限性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号