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首页> 外文期刊>Biochemistry >A novel l-xylulose reductase essential for l-arabinose catabolism in trichoderma reesei
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A novel l-xylulose reductase essential for l-arabinose catabolism in trichoderma reesei

机译：一种新的l-木酮糖还原酶对里氏木霉的l-阿拉伯糖分解代谢至关重要

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摘要

l-Xylulose reductases belong to the superfamily of short chain dehydrogenases and reductases (SDRs) and catalyze the NAD(P)H-dependent reduction of l-xylulose to xylitol in l-arabinose and glucuronic acid catabolism. Here we report the identification of a novel l-xylulose reductase LXR3 in the fungus Trichoderma reesei by a bioinformatic approach in combination with a functional analysis. LXR3, a 31 kDa protein, catalyzes the reduction of l-xylulose to xylitol via NADPH and is also able to convert d-xylulose, d-ribulose, l-sorbose, and d-fructose to their corresponding polyols. Transcription of lxr3 is specifically induced by l-arabinose and l-arabitol. Deletion of lxr3 affects growth on l-arabinose and l-arabitol and reduces total NADPH-dependent LXR activity in cell free extracts. A phylogenetic analysis of known l-xylulose reductases shows that LXR3 is phylogenetically different from the Aspergillus nigerl-xylulose reductase LxrA and, moreover, that all identified true l-xylulose reductases belong to different clades within the superfamily of SDRs. This indicates that the enzymes responsible for the reduction of l-xylulose in l-arabinose and glucuronic acid catabolic pathways have evolved independently and that even the fungal LXRs of the l-arabinose catabolic pathway have evolved in different clades of the superfamily of SDRs.
机译：1-木酮糖还原酶属于短链脱氢酶和还原酶（SDR）的超家族，并在1-阿拉伯糖和葡萄糖醛酸分解代谢中催化NAD（P）H依赖性的1-木酮糖还原为木糖醇。在这里，我们报告通过生物信息学方法结合功能分析，在里氏木霉属真菌中鉴定出一种新型的l-木酮糖还原酶LXR3。 LXR3是一种31 kDa的蛋白质，可通过NADPH催化将L-木酮糖还原为木糖醇，并且还能够将D-木酮糖，D-核糖，L-山梨糖和D-果糖转化为其相应的多元醇。 1-阿拉伯糖和1-阿拉伯糖醇特异性地诱导1xr3的转录。 lxr3的缺失会影响l-阿拉伯糖和l-阿拉伯糖醇的生长，并降低无细胞提取物中总NADPH依赖性LXR活性。已知的l-木酮糖还原酶的系统发育分析表明，LXR3与黑曲霉的木糖还原酶LxrA在系统发育上不同，此外，所有鉴定出的真正的l-木酮糖还原酶都属于SDR超家族中的不同进化枝。这表明负责减少1-阿拉伯糖和葡萄糖醛酸分解代谢途径中的1-木酮糖的酶已经独立进化，并且甚至1-阿拉伯糖分解代谢途径的真菌LXR也已经在SDR超家族的不同进化枝中进化。

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