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首页> 外文期刊>Acta Horticulturae >Mapping of QTLs for yield and alpha acid content in hop.
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Mapping of QTLs for yield and alpha acid content in hop.

机译：QTL的产量和啤酒花中α酸含量的映射。

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摘要

Hop breeding is a lengthy process due to the dioecious nature of hop, producing highly heterozygous offspring and only female plants are of commercial interest. Molecular approaches have therefore been developed to support conventional hop breeding programmes. We studied the effects of quantitative trait loci (QTLs) and determined map locations for alpha-acid content and yield in hop, using amplified fragment length polymorphism (AFLP) and microsatellite markers (SSRs). Genetic linkage maps were constructed from a mapping population consisting of 111 progeny from a double pseudo-testcross. A total of 194 markers were located on the 20 linkage groups (LGs) of the maternal and paternal maps, covering total map lengths of 706 and 616 cM, respectively. Due to the presence of common biparental SSR markers, homology of LGs between parental maps could be inferred. The progeny segregated quantitatively for alpha-acid content and yield determined in the years from 2002-2006. Several putative QTLs were determined and the results are discussed from the point of view of implementation of a marker-assisted selection (MAS) programme in hop.
机译：由于啤酒花的雌雄异体，酒花育种是一个漫长的过程，会产生高度杂合的后代，只有雌性植物才具有商业价值。因此，已经开发出分子方法来支持常规啤酒花育种程序。我们使用扩增的片段长度多态性（AFLP）和微卫星标记（SSR）研究了数量性状基因位点（QTL）的影响，并确定了啤酒花中α-酸含量和产量的定位图。遗传连锁图谱由来自双重假testcross的111个子代组成的作图群体构建。总共194个标记位于母本和父本图谱的20个连锁组（LG）上，分别覆盖了706 cM和616 cM的总图谱长度。由于存在常见的双亲SSR标记，因此可以推断出亲本图谱之间LG的同源性。后代定量分离了2002-2006年间测定的α-酸含量和产量。确定了几个推定的QTL，并从啤酒花中标记辅助选择（MAS）程序的实施角度讨论了结果。

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