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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Molecular Response of Human Monocytes Following Interaction with Colon Cancer Cells by Pre-treatment with Low-dose Lipopolysaccharide
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Molecular Response of Human Monocytes Following Interaction with Colon Cancer Cells by Pre-treatment with Low-dose Lipopolysaccharide

机译：通过低剂量脂多糖预处理与结肠癌细胞相互作用后人类单核细胞的分子反应

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Background/Aim: The increased mRNA expression of chemotaxis-and angiogenesis-related factors in human monocytes following interaction with colon cancer cells has been shown to be suppressed by pre-treatment with low-dose lipopolysaccharide (LPS) (100 pg/ml). It has been demonstrated that low-dose LPS reduced the expression of RelB, a member of the nuclear factor (NF)-kappa B transcription factor family, in mouse macrophages and the NF-kappa B signaling pathway was important for tumor initiation and growth in tumor-associated macrophages. In addition, the signal transducer and activator of transcription 3 (STAT3) regulated innate immunity via Toll-like receptor (TLR) 4 signaling. In the present study, the mRNA expression of signaling pathway-and suppression-related genes in human monocytes following a low-dose LPS treatment and subsequent interaction with colon cancer cells was investigated, in order to assess the molecular response. Materials and Methods: The human monocyte cell line THP-1 was treated with LPS and, subsequently, co-cultured with the human colon cancer cell line DLD-1. The mRNA expression of various genes was then analyzed using quantitative real-time polymerase chain reaction (PCR). Results: The mRNA expression of RelB, STAT3, interleukin (IL)-10 and transforming growth factor (TGF)-beta in THP-1 cells following interaction with DLD-1 cells was suppressed by pre-treatment with low-dose LPS (100 pg/ml). Conclusion: Treating human monocytes with low-dose LPS may be useful for suppressing tumor progression and may be valuable for maintaining homeostasis.
机译：背景/目的：通过低剂量脂多糖（LPS）（100 pg / ml）的预处理可以抑制与结肠癌细胞相互作用后人单核细胞中趋化性和血管生成相关因子的mRNA表达增加。已经证明低剂量的LPS会降低小鼠巨噬细胞中RelB（核因子（NF）-κB转录因子家族的成员）的表达，并且NF-κB信号通路对于肿瘤的发生和生长很重要。肿瘤相关巨噬细胞。此外，信号转导和转录激活因子3（STAT3）通过Toll样受体（TLR）4信号传导调节先天免疫。在本研究中，研究了低剂量LPS​​处理后人单核细胞中信号通路和抑制相关基因的mRNA表达以及随后与结肠癌细胞的相互作用，以评估分子反应。材料和方法：用LPS处理人单核细胞系THP-1，然后与人结肠癌细胞系DLD-1共培养。然后使用定量实时聚合酶链反应（PCR）分析各种基因的mRNA表达。结果：低剂量脂多糖预处理可抑制THP-1细胞与DLD-1细胞相互作用后RelB，STAT3，白介素（IL）-10和转化生长因子（TGF）-β的mRNA表达（100）。 pg / ml）。结论：低剂量LPS​​治疗人单核细胞可能对抑制肿瘤进展有用，对于维持体内稳态也可能具有重要价值。

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