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首页> 外文期刊>Nanotechnology >Investigation into local cell mechanics by atomic force microscopy mapping and optical tweezer vertical indentation
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Investigation into local cell mechanics by atomic force microscopy mapping and optical tweezer vertical indentation

机译：通过原子力显微镜作图和光学镊子垂直压痕研究局部细胞力学

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Investigating the mechanical properties of cells could reveal a potential source of label-free markers of cancer progression, based on measurable viscoelastic parameters. The Young's modulus has proved to be the most thoroughly studied so far, however, even for the same cell type, the elastic modulus reported in different studies spans a wide range of values, mainly due to the application of different experimental conditions. This complicates the reliable use of elasticity for the mechanical phenotyping of cells. Here we combine two complementary techniques, atomic force microscopy (AFM) and optical tweezer microscopy (OTM), providing a comprehensive mechanical comparison of three human breast cell lines: normal myoepithelial (HBL-100), luminal breast cancer (MCF-7) and basal breast cancer (MDA-MB-231) cells. The elastic modulus was measured locally by AFM and OTM on single cells, using similar indentation approaches but different measurement parameters. Peak force tapping AFM was employed at nanonewton forces and high loading rates to draw a viscoelastic map of each cell and the results indicated that the region on top of the nucleus provided the most meaningful results. OTM was employed at those locations at piconewton forces and low loading rates, to measure the elastic modulus in a real elastic regime and rule out the contribution of viscous forces typical of AFM. When measured by either AFM or OTM, the cell lines' elasticity trend was similar for the aggressive MDA-MB-231 cells, which were found to be significantly softer than the other two cell types in both measurements. However, when comparing HBL-100 and MCF-7 cells, we found significant differences only when using OTM.
机译：基于可测量的粘弹性参数，研究细胞的机械性质可以揭示癌症发展过程中无标记的标志物的潜在来源。迄今为止，杨氏模量被证明是研究最彻底的，但是，即使对于相同的细胞类型，不同研究报告的弹性模量也涵盖了很宽的数值范围，这主要是由于应用了不同的实验条件。这使可靠地利用弹性进行细胞的机械表型化变得复杂。在这里，我们结合了原子力显微镜（AFM）和光学镊子显微镜（OTM）两种互补技术，对三种人类乳腺细胞系进行了全面的机械比较：正常肌上皮细胞（HBL-100），腔内乳腺癌（MCF-7）和基底乳腺癌（MDA-MB-231）细胞。弹性模量是通过AFM和OTM在单个电池上使用相似的压痕方法，但测量参数不同来局部测量的。在纳米牛顿力和高加载速率下使用峰值力攻丝AFM绘制每个细胞的粘弹性图，结果表明，细胞核顶部的区域提供了最有意义的结果。 OTM在微微力和低加载速率下的那些位置使用，以测量实际弹性状态下的弹性模量，并排除AFM典型的粘性力的作用。当通过AFM或OTM进行测量时，侵略性MDA-MB-231细胞的细胞系弹性趋势相似，发现这两种方法均明显比其他两种细胞柔软。但是，在比较HBL-100和MCF-7细胞时，我们仅在使用OTM时才发现显着差异。

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