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Subdiffraction scattered light imaging of gold nanoparticles using structured illumination

机译:使用结构化照明的金纳米粒子的亚衍射散射光成像

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摘要

A reflective light-scattering (RLS) microscope with structured illumination (SI) provides subdiffraction resolution and improves the image quality of gold nanoparticles in biological systems. The three-dimensional (3D)-structured pattern is rapidly and precisely controlled with a spatial light modulator and scrambled at the conjugate image plane to increase spatial incoherence. The reconstructed SI-RLS image of 100 nm gold nanoparticles reveals lateral and axial resolutions of approximately 117 and 428 nm. We present a high-resolution image of gold nanoparticles inside a HeLa cell, with improved contrast.
机译:具有结构化照明(SI)的反射光散射(RLS)显微镜可提供亚衍射分辨率,并改善了生物系统中金纳米颗粒的图像质量。三维(3D)结构的图案可通过空间光调制器快速精确地控制,并在共轭像面上加扰以增加空间不相干性。 100 nm金纳米颗粒的重建SI-RLS图像显示出大约117和428 nm的横向和轴向分辨率。我们提出了HeLa细胞内部的金纳米颗粒的高分辨率图像,具有改善的对比度。

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