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Low photobleaching and high emission depletion efficiency: the potential of AIE luminogen as fluorescent probe for STED microscopy

机译:低光漂白和高发射损耗效率:AIE发光剂作为STED显微镜荧光探针的潜力

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摘要

We present a preliminary study which explores the potential of aggregation-induced emission (AIE) luminogen as a new fluorescent probe for STED microscopy. Compared with Coumarin 102, which is a commonly used organic fluorophore in STED microscopy, HPS, a typical AIE luminogen, is more resistant to photobleaching. In addition, HPS-doped nanoparticles have higher emission depletion efficiency than Coumarin 102 in organic solution. These two advantages of AIE luminogen can facilitate the improvement of spatial resolution, as well as long-term imaging, in STED microscopy. AIE luminogen will be a promising candidate for STED microscopy in the future. (C) 2015 Optical Society of America
机译:我们提出了一项初步研究,探讨了潜在的聚集诱导发射(AIE)发光剂作为STED显微镜的新型荧光探针的潜力。与在STED显微镜中常用的有机荧光体香豆素102相比,典型的AIE发光剂HPS更耐光漂白。此外,在有机溶液中,掺有HPS的纳米颗粒比香豆素102具有更高的发射损耗效率。 AIE发光剂的这两个优点可以促进STED显微镜中空间分辨率的改善以及长期成像。 AIE发光原将在将来成为STED显微镜的有希望的候选者。 (C)2015年美国眼镜学会

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