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首页> 外文期刊>Cornea >Short-term corneal response to cross-linking in rabbit eyes assessed by in vivo confocal laser scanning microscopy and histology.
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Short-term corneal response to cross-linking in rabbit eyes assessed by in vivo confocal laser scanning microscopy and histology.

机译：通过体内共聚焦激光扫描显微镜和组织学评估兔眼对交联的短期角膜反​​应。

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PURPOSE: Corneal cross-linking for the treatment of keratoconus has been tested in animal trials and proven clinically. A combination of in vivo confocal laser scanning microscopy (CLSM) and histology was used in rabbit corneas to assess early modifications at the cellular level after corneal cross-linking. METHODS: Twelve New Zealand male rabbits were tested; in each case, the right eye was the study eye and left eye was the control eye. In vivo CLSM was performed on both eyes before and at 3 days and 1 week after cross-linking. Keratocyte and endothelial cell densities were determined by CLSM before and after cross-linking. After CLSM, the corneas were excised and processed for histology and immunohistochemistry. RESULTS: Massive edema was observed 3 days after cross-linking. The corneal epithelium had already closed again by day 3. No cellular structures were detected in the stroma and endothelium. One week after cross-linking, normal corneal transparency and thickness were restored. The anterior stroma still lacked nuclei. The number of nuclei in the posterior stroma was significantly lower than that in the intact corneas. Highly reflective spindle-shaped structures were detected in the posterior stroma. The endothelial monolayer had closed again but still showed significantly decreased cell density. At 1 week after cross-linking, immunohistochemical staining revealed the presence of proliferating cells in the corneal epithelium, posterior stroma, and endothelium. CONCLUSIONS: The early response of the rabbit cornea to cross-linking was successfully characterized at the cellular level by in vivo CLSM and histology, and the results obtained with both techniques correlated positively.
机译：目的：角膜交联用于治疗圆锥角膜已经在动物试验中进行了测试，并得到临床证明。体内共聚焦激光扫描显微镜（CLSM）和组织学的组合用于兔角膜，以评估角膜交联后细胞水平的早期修饰。方法：对12只新西兰雄性兔进行了测试;在每种情况下，右眼为研究眼，左眼为对照眼。在交联之前以及交联后3天和1周在两只眼睛上进行体内CLSM。在交联之前和之后通过CLSM确定角质形成细胞和内皮细胞的密度。 CLSM后，切除角膜并进行组织学和免疫组织化学处理。结果：交联后3天观察到大量水肿。到第3天，角膜上皮已经再次关闭。在基质和内皮中未检测到细胞结构。交联后一周，角膜透明度和厚度恢复正常。前间质仍缺乏核。后基质的细胞核数目明显少于完整角膜的细胞核数目。在后基质中检测到高反射纺锤形结构。内皮单层再次关闭，但仍显示细胞密度明显降低。交联后第1周，免疫组织化学染色显示角膜上皮，后基质和内皮中存在增殖细胞。结论：通过体内CLSM和组织学成功地在细胞水平上表征了兔角膜对交联的早期反应，并且两种技术获得的结果均呈正相关。

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