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Diversity analysis of Sesbania acculeata by using transferable rice SSR markers.

机译：利用可转移水稻SSR标记分析 Sesbania acculeata 的多样性。

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摘要

In this experiment, the transferability of 13 rice (Oryza sativa) simple sequence repeats (SSR) loci to Sesbania acculeata (27 accessions) was studied. Upto 17.5% of the rice micro satellite primer pairs amplified SSRs within S. acculeata. The highest level of successful with a single primer (RM 6160) was 40.7% for S. acculeata. The transferability of these SSR primers indicated a high level of sequence conservation in these regions across genus even taxa. Together with their locus-specificity, co-dominant nature and potential to amplify multiple alleles, their transferability makes simple sequence repeats marker a powerful tool for genetic mapping, diversity analysis and genotyping. Knowledge of DNA sequence is essential before SSR loci can be meaningfully used to address applied and evolutionary questions. The true benefit of the transfer of these markers between species lies in the reduced need to undergo expensive and labour intensive methodology to develop genus-specific SSR marker.
机译：在该实验中，研究了13个水稻（S.Oryza sativa ）简单序列重复（SSR）位点对Sesbania acculeata （27种）的可转移性。最多有17.5％的水稻微卫星引物对在S内扩增了SSR。 单个引物（RM 6160）的最高成功率为i.S 40.7％。 这些SSR引物的可转移性表明，整个类群中这些区域的序列保守性都很高。加上其基因座特异性，共同优势的性质以及扩增多个等位基因的潜力，它们的可转移性使简单的序列重复标记成为遗传绘图，多样性分析和基因分型的强大工具。必须先了解DNA序列，然后才能将SSR基因座有效地用于解决应用问题和进化问题。这些标记在物种之间转移的真正好处在于，减少了开发昂贵的SSR标记所需的昂贵且费力的方法。

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