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首页> 外文期刊>Current Biology: CB >Artificial tethering to nuclear pores promotes partitioning of extrachromosomal DNA during yeast asymmetric cell division.
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Artificial tethering to nuclear pores promotes partitioning of extrachromosomal DNA during yeast asymmetric cell division.

机译：在酵母非对称细胞分裂过程中，对核孔的人工拴系促进染色体外DNA的分配。

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摘要

Asymmetric cell division in unicellular organisms enables sequestration of senescence factors to specific subpopulations. Accumulation of autonomously replicating sequence (ARS) plasmids, which frequently emerge from recombination within the highly repetitive ribosomal DNA locus, is linked to limited replicative life span of Saccharomyces cerevisiae cells [1]. During budding yeast cell division, ARS plasmids are retained in the ageing mother cell, such that only 1 out of 10 plasmids enters the rejuvenated bud [2]. Binding of ARS plasmids to nuclear structures retained in the mother cell was speculated to explain asymmetric plasmid segregation [2]. Association with nuclear pore complexes (NPCs) was proposed to underlie retention of ARS plasmids in the mother cell [3]. However, the role of NPCs in segregation of ARS plasmids is unclear, as NPCs are partitioned between mother and bud nuclei during mitosis [4,5]. Here we analyzed how segregation of ARS plasmids is influenced by their interaction with NPCs. We found that artificial tethering to NPCs promotes transport of ARS plasmids into the bud. Moreover, our experiments provide support for the notion that interaction with ARS plasmids does not affect movement of NPCs into the bud. We conclude that binding to NPCs cannot by itself contribute to asymmetric segregation of ARS plasmids.
机译：单细胞生物中的不对称细胞分裂能够将衰老因子隔离到特定的亚群。高度复制的核糖体DNA基因座中重组经常出现的自主复制序列（ARS）质粒的积累与酿酒酵母细胞的有限复制寿命有关[1]。在酵母细胞芽分化过程中，ARS质粒保留在衰老的母细胞中，因此10个质粒中只有1个进入再生的芽[2]。据推测，ARS质粒与保留在母细胞中的核结构的结合可以解释不对称质粒的分离[2]。有人提出与核孔复合物（NPC）的结合是将ARS质粒保留在母细胞中的基础[3]。然而，尚不清楚NPC在ARS质粒分离中的作用，因为在有丝分裂期间NPC在母核和芽核之间分配[4,5]。在这里，我们分析了ARS质粒与NPC的相互作用如何影响其分离。我们发现对NPC进行人工系链可促进ARS质粒向芽中的转运。此外，我们的实验为与ARS质粒相互作用不会影响NPC进入芽中的观点提供了支持。我们得出结论，与NPC的结合本身不能促进ARS质粒的不对称分离。

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