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首页> 外文期刊>Vaccine >Protective and antifecundity effects of Sm-p80-based DNA vaccine formulation against Schistosoma mansoni in a nonhuman primate model
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Protective and antifecundity effects of Sm-p80-based DNA vaccine formulation against Schistosoma mansoni in a nonhuman primate model

机译：在非人类灵长类动物模型中，基于Sm-p80的DNA疫苗制剂对曼氏血吸虫的保护和抗生殖力作用

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Schistosomiasis is an important parasitic disease for which there is no available vaccine. We have focused on a functionally important antigen of Schistosoma mansoni, Sm-p80, as a vaccine candidate because of its consistent immunogenicity, protective potential and antifecundity effect observed in murine models; and for its pivotal role in the immune evasion process. In the present study we report that an Sm-p80-based DNA vaccine formulation confers 38% reduction in worm burden in a nonhuman primate model, the baboon (Papio anubis). Animals immunized with Sm-p80-pcDNA3 exhibited a decrease in egg production by 32%. Sm-p80 DNA elicited specific immune responses that include IgG; its subtypes IgG1 and IgG2; and IgM in vaccinated animals. Peripheral blood mononuclear cells (PBMCs) from immunized animals when stimulated in vitro with Sm-p80 produced appreciably more Th1 response enhancing cytokines (IL-2, IFN-gamma) than Th2 response enhancing cytokines (IL-4, IL-10). PBMCs produced appreciably more spot-forming units for INF-gamma than for IL-4 in enzyme-linked immunosorbent spot (ELISPOT) assays. Overall it appears that even though a mixed (Th1/Th2) type of humoral antibody response was generated following immunization with Sm-p80; the dominant protective immune response is Th1 type. These data reinforce the potential of Sm-p80 as an excellent vaccine candidate for schistosomiasis.
机译：血吸虫病是一种重要的寄生虫病，没有可用的疫苗。我们已经将曼氏血吸虫的功能重要抗原Sm-p80作为候选疫苗，因为它在鼠模型中观察到了一致的免疫原性，保护潜力和抗生殖力作用。并在免疫逃避过程中起关键作用。在本研究中，我们报告了基于Sm-p80的DNA疫苗制剂可在非人类灵长类动物模型狒狒（Papio anubis）中使蠕虫负担减少38％。用Sm-p80-pcDNA3免疫的动物产卵量减少了32％。 Sm-p80 DNA引发了包括IgG在内的特异性免疫反应。其亚型IgG1和IgG2;接种动物中的IgM。当用Sm-p80体外刺激时，来自免疫动物的外周血单核细胞（PBMC）产生的Th1反应增强细胞因子（IL-2，IFN-γ）比Th2反应增强细胞因子（IL-4，IL-10）多得多。在酶联免疫吸附斑点法（ELISPOT）中，PBMC产生的INF-γ斑点形成单位明显多于IL-4。总体看来，即使在用Sm-p80免疫后产生了混合的（Th1 / Th2）类型的体液抗体应答;但是，似乎没有发生。主要的保护性免疫反应是Th1型。这些数据增强了Sm-p80作为血吸虫病优良疫苗候选者的潜力。

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