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首页> 外文期刊>Vaccine >Vaccination with recombinant Clostridium perfringens toxoids alpha and beta promotes elevated antepartum and passive humoral immunity in swine
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Vaccination with recombinant Clostridium perfringens toxoids alpha and beta promotes elevated antepartum and passive humoral immunity in swine

机译：用重组产气荚膜梭菌类毒素α和β进行疫苗接种可提高猪的产前和被动体液免疫

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摘要

Due to the increasingly restricted use of antimicrobials in animal production systems, the prevention and control of Clostridium perfringens type A- and C-induced diarrhea in piglets should be based on passive immunization via the prepartum vaccination of sows. Given the current obstacles in the production of conventional clostridial vaccines, the use of recombinant proteins has been considered to represent a promising alternative. In the present study, the neutralizing antibody response of immunized sows and their litters to a bivalent vaccine containing the C. perfringens recombinant toxoids alpha (rTA) and beta (rTB) produced in Escherichia coli was assessed. Rabbits (n = 8) and pregnant sows (n = 7) were immunized with 200 mu g of each recombinant antigen using Al(OH)(3) as adjuvant. The alpha and beta antitoxin titer detected in the rabbits' serum pool was 9.6 and 20.4 IU/mL, respectively. The mean alpha and beta antitoxin titers in the sows' sera were 6.0 +/- 0.9 IU/mL and 14.5 +/- 2.2 IU/mL, and the corresponding individual coefficients of variation (CV) were 16.04% and 14.91%, respectively. The mean alpha and beta antitoxin titers in the litters' serum pools were 4.2 +/- 0.4 IU/mL and 10.9 +/- 1.7 IU/mL, and the CV between litters was 9.23% and 9.85%, respectively. The results showed that the rTA and rTB proteins produced and tested in the present study induced an immune response and can be regarded as candidates for the development of a commercial vaccine against C perfringens type A- and C-induced diarrhea in pigs.
机译：由于动物生产系统中抗菌药物的使用日益受到限制，因此预防和控制猪产气荚膜梭状芽胞杆菌A型和C型腹泻的预防和控制应基于母猪产前疫苗的被动免疫。鉴于目前常规梭菌疫苗生产中的障碍，重组蛋白质的使用被认为是一种有前途的替代方法。在本研究中，评估了免疫母猪及其仔猪对含有在大肠杆菌中产生的产气荚膜梭菌重组类毒素α（rTA）和β（rTB）的二价疫苗的中和抗体应答。兔（n = 8）和母猪（n = 7）用Al（OH）（3）作为佐剂用200μg的每种重组抗原免疫。在兔子的血清库中检测到的α和β抗毒素效价分别为9.6和20.4 IU / mL。母猪血清中的平均α和β抗毒素效价为6.0 +/- 0.9 IU / mL和14.5 +/- 2.2 IU / mL，相应的个体变异系数（CV）分别为16.04％和14.91％。产仔血清库中平均α和β抗毒素滴度为4.2 +/- 0.4 IU / mL和10.9 +/- 1.7 IU / mL，产仔之间的CV分别为9.23％和9.85％。结果表明，在本研究中生产和测试的rTA和rTB蛋白诱导了免疫反应，可以被视为开发针对猪产气荚膜杆菌A型和C型腹泻的商业疫苗的候选药物。

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