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首页> 外文期刊>Vaccine >Relation of activation-induced deaminase (AID) expression with antibody response to A(H1N1)pdm09 vaccination in HIV-1 infected patients
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Relation of activation-induced deaminase (AID) expression with antibody response to A(H1N1)pdm09 vaccination in HIV-1 infected patients

机译：HIV-1感染患者中激活诱导的脱氨酶（AID）表达与对A（H1N1）pdm09疫苗接种的抗体应答的关系

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摘要

The relevance of CD4+T-cells, viral load and age in the immunological response to influenza infection and vaccination in HIV-1 infected individuals has previously been pointed out. Our study aimed at assessing, in the setting of 2009 A(H1N1)pdm09 influenza vaccination, whether quantification of activation-induced deaminase (AID) expression in blood B-cells may provide additional indications for predicting antibody response to vaccination in HIV-1 infected patients with similar CD4+T-cell counts and age. Forty-seven healthy controls, 37 ART-treated and 17 treatment-naive HIV-1 infected patients were enrolled in the study. Blood was collected prior to A(H1N1)pdm09 vaccination and at 1, 3 and 6 months after vaccination. Antibody titers to A(H1N1)pdm09 vaccine were measured by hemagglutination inhibition (HI) assay while the mRNA expression levels of AID were measured by quantitative real time PCR. Upon B-cell activation in vitro, AID increase correlated to antibody response to the A(H1N1)pdm09 vaccine at 1 month after vaccination in all individuals. In addition, the maximum expression levels of AID were significantly higher in those individuals who still carried protective levels of A(H1N1)pdm09 antibodies after 6 months from vaccination. No correlation was found between CD4+T-cell counts or age at vaccination or HIV-1 viral load and levels of A(H1N1)pdm09 antibodies. Assessing AID expression before vaccination may be an additional useful tool for defining a vaccination strategy in immune-compromised individuals at risk of immunization failure
机译：先前已经指出，CD4 + T细胞，病毒载量和年龄与HIV-1感染者对流感病毒感染和疫苗接种的免疫反应有关。我们的研究旨在评估在2009年A（H1N1）pdm09流感疫苗接种中，血液B细胞中活化诱导的脱氨酶（AID）表达的量化是否可能为预测HIV-1感染者对疫苗接种的抗体反应提供其他指示CD4 + T细胞计数和年龄相似的患者。该研究纳入了47名健康对照，37名接受ART治疗的患者和17名未经治疗的HIV-1感染患者。在接种A（H1N1）pdm09之前以及接种后1、3和6个月收集血液。 A（H1N1）pdm09疫苗的抗体滴度通过血凝抑制（HI）测定，而AID的mRNA表达水平通过实时定量PCR测定。在体外B细胞激活后，所有个体中疫苗接种后1个月，AID的增加与对A（H1N1）pdm09疫苗的抗体反应相关。此外，在接种疫苗6个月后仍携带保护性A（H1N1）pdm09抗体的个体中，AID的最大表达水平明显更高。在CD4 + T细胞计数或疫苗接种年龄或HIV-1病毒载量与A（H1N1）pdm09抗体水平之间未发现相关性。在疫苗接种之前评估AID表达可能是在有免疫接种失败风险的免疫受损个体中定义疫苗接种策略的另一种有用工具

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