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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Antioxidant and antiproliferative activities of spirulina and chlorella water extracts
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Antioxidant and antiproliferative activities of spirulina and chlorella water extracts

机译：螺旋藻和小球藻水提取物的抗氧化和抗增殖活性

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Liver fibrosis is a chronic liver disease that will further develop to cirrhosis if severe damage continues to form. A potential treatment for liver fibrosis is to inhibit activated hepatic stellate cell (HSC) proliferation and, subsequently, to induce HSC apoptosis. It has been reported that antioxidants are able to inhibit the proliferation of HSCs. In this study, the aqueous extract of spirulina was chosen as the source of antioxiclant to investigate the inhibitory effect on the proliferation of HSC. The growth inhibitory effects of aqueous spirulina and chlorella extract on human liver cancer cells, HepG2, were also studied and compared in pairs. Results indicated that the total phenol content of spirulina was almost five times greater than that of chlorella (6.86 ± 10.58 vs 1.44 ± 0.04 mg tannic acid equivalent/g of algae powder, respectively). The antioxiclant activity of spirulina determined by the ABTS(•+) method was higher than chlorella (EC50: 72.44 ± 0.24 umol of trolox equivalent/g of spirulina extract vs 56.09 ± 1.99 μ mol of trolox equivalent/g of chlorella extract). Results of DPPH• assay also showed a similar trend as the ABTS(•+) assay (EC50: 19.39 ± 0.65 μ mol of ascorbic acid equivalent/g of spirulina extract vs 14.04 ± 1.06 μ mol of ascorbic acid equivalent/g of chlorella extract). The aqueous extracts of these two algae both showed antiproliferative effects on HSC and HepG2, but spirulina was a stronger inhibitor than chlorella. Annexin-V staining showed that aqueous extract of spirulina induced apoptosis of HSC after 12 h of treatment. In addition, the aqueous extract of spirulina triggered a cell cycle arrest of HSC at the G2/M phase.
机译：肝纤维化是一种慢性肝病，如果继续形成严重损害，将进一步发展为肝硬化。肝纤维化的潜在治疗方法是抑制肝星状细胞（HSC）的活化增殖，然后诱导HSC凋亡。据报道，抗氧化剂能够抑制HSC的增殖。在这项研究中，选择螺旋藻的水提取物作为抗氧剂的来源，以研究其对HSC增殖的抑制作用。螺旋藻和小球藻提取物对人肝癌细胞HepG2的生长抑制作用也进行了研究并成对比较。结果表明，螺旋藻的总酚含量几乎比小球藻高五倍（分别为6.86和PLUSMN; 10.58与1.44和PLUSMN; 0.04 mg单宁酸当量/每克藻粉）。通过ABTS（＆BULL; +）方法测定的螺旋藻的抗氧化活性高于小球藻（EC50：72.44＆PLUSMN;相对于每克螺旋藻提取物0.24 umol vs.56.09＆PLUSMN;相对于每克小球藻提取物1.99μmol相对于小球藻）。 DPPH＆BULL的结果;测定也显示出与ABTS（＆BULL; +）测定相似的趋势（EC50：19.39＆PLUSMN; 0.65μmol抗坏血酸当量/ g螺旋藻提取物vs 14.04＆PLUSMN; 1.06μmol抗坏血酸当量/ g小球藻提取物） 。这两种藻类的水提取物均对HSC和HepG2表现出抗增殖作用，但螺旋藻比小球藻更强。 Annexin-V染色显示螺旋藻的水提取物在处理12小时后可诱导HSC凋亡。另外，螺旋藻的水提取物触发了HSC在G2 / M期的细胞周期停滞。

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