...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Agricultural and Food Chemistry >Collaborative Validation of an Event-Specific Quantitative Real-Time PCR Method for Genetically Modified Rice Event TT51-1 Detection
【24h】

Collaborative Validation of an Event-Specific Quantitative Real-Time PCR Method for Genetically Modified Rice Event TT51-1 Detection

机译：事件特异性定量实时PCR方法用于转基因水稻事件TT51-1检测的协同验证

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, a collaborative trial of validating a real-time PCR method for the TT51-1 rice event was organized, including six participating laboratories. In this validation, serially diluted solutions from homogeneous genomic DNA of the TT51-1 event were used to construct standard curves of the TT51-1 event and phospholipase D (PLD) assays. The PCR efficiency was 95%, and the R2 coefficient was 0.99 for the TT51-1 system. The mean quantitative values for blind samples containing 0.1%, 0.5% 1%, 5%, and 10% (w/w) TT51-1 corresponded to 0.1%, 0.51%, 1.06%, 4.83%, and 9.62%, respectively, with a bias (%) ranging from -3.77% to 5.87%. The repeatability and reproducibility were all below 25% across the entire dynamic range. Furthermore, the measurement uncertainties of the quantitative results were estimated to be 0.10%, 0.20%, 0.40%, 1.76%, and 3.52% (w/w) for the tested samples. Both the LOD and LOQ were calculated to be 0.22%. This collaborative trial demonstrated that the TT51-1 method produces reliable, comparable, and reproducible results for a given sample set and can be adopted as a detection standard for testing laboratories.
机译：在这项研究中，组织了一项协作试验，以验证TT51-1水稻事件的实时PCR方法，包括六个参与实验室。在此验证中，使用了来自TT51-1事件的同质基因组DNA的系列稀释溶液来构建TT51-1事件和磷脂酶D（PLD）分析的标准曲线。对于TT51-1系统，PCR效率为95％，R2系数为0.99。含0.1％，0.5％，1％，5％和10％（w / w）TT51-1的盲样品的平均定量值分别对应于0.1％，0.51％，1.06％，4.83％和9.62％。偏差（％）在-3.77％至5.87％之间。在整个动态范围内，重复性和再现性均低于25％。此外，对于测试样品，定量结果的测量不确定度估计为0.10％，0.20％，0.40％，1.76％和3.52％（w / w）。计算出的LOD和LOQ均为0.22％。这项合作性试验证明，对于给定的样本集，TT51-1方法可产生可靠，可比较和可重现的结果，并且可以用作测试实验室的检测标准。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号