首页> 外文期刊>Journal of Agricultural and Food Chemistry >Characterization of Proanthocyanidins from Seeds of Perennial Ryegrass (Lolium perenne L.) and Tall Fescue (Festuca arundinacea) by Liquid Chromatography-Mass Spectrometry
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Characterization of Proanthocyanidins from Seeds of Perennial Ryegrass (Lolium perenne L.) and Tall Fescue (Festuca arundinacea) by Liquid Chromatography-Mass Spectrometry

机译:液相色谱-质谱法鉴定多年生黑麦草(Lolium perenne L.)和高羊茅(Festuca arundinacea)种子中的原花色素

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摘要

Perennial ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea) are forage species of the grass family (Poaceae) that are key components of temperate pasture-based agricultural systems. Proanthocyanidins (PAs) are oligomeric flavonoids that, when provided as part of a farm animal's diet, have been reported to improve animal production and health. Up to now, forage grasses have been deemed not to produce PAs. This paper reports for the first time the detection of polymerized PAs in aqueous methanolic extracts of seed tissue of both perennial ryegrass and tall fescue, using LC-MS/MS. We have determined the structure of the PAs to be trans-flavan-3-ol-based, consisting predominately of afzelechin and catechin and linked primarily by B-type bonds. Investigations into the leaf tissue of both species failed to detect any PAs. This discovery opens the possibility of using genetic engineering tools to achieve tannin accumulation in leaf tissue of perennial ryegrass and tall fescue.
机译:多年生黑麦草(Lolium perenne)和高羊茅(Festuca arundinacea)是草科(禾本科)的牧草物种,是温带牧场型农业系统的重要组成部分。原花青素(PAs)是一种低聚类黄酮,据报道,这种黄酮作为农场动物饮食的一部分提供时,可以改善动物的生产和健康。到目前为止,草料草被认为不产生PA。本文首次报道了使用LC-MS / MS检测多年生黑麦草和高羊茅种子组织的甲醇提取液中的聚合PAs。我们已经确定PA的结构是基于反黄酮-3-醇的,主要由afzelechin和儿茶素组成,并且主要通过B型键连接。对这两个物种的叶子组织的调查均未发现任何PA。这一发现为利用基因工程工具实现多年生黑麦草和高羊茅叶片组织中单宁的积累提供了可能性。

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