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首页> 外文期刊>Journal of Applied Polymer Science >Reduction of Free Radicals and Endotoxin by Conjugated Linoleic Acid Loaded in an In Situ-Synthesized Poly(N-isopropyl acrylamide) Thin Layer
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Reduction of Free Radicals and Endotoxin by Conjugated Linoleic Acid Loaded in an In Situ-Synthesized Poly(N-isopropyl acrylamide) Thin Layer

机译：负载在原位合成聚（N-异丙基丙烯酰胺）薄层中的共轭亚油酸对自由基和内毒素的还原作用

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A thin layer of poly(N-isopropyl acrylamide) (pNIPAAm) was synthesized in situ on the surface of hydrolyzed polyacrylonitrile (PAN) membrane. This thin layer exhibited both pH response due to the poly(acrylic acid) moiety and temperature response due to the pNIPAAm moiety. The swelling behavior of the membranes was evaluated under various temperatures and pH. The curve of the swelling ratio for the PAN-NIPAAm showed a lower critical solution temperature. Then, conjugated linoleic acid (CLA) was loaded into the pNIPAAm layer. The effects of CLA on the blood coagulation and oxidative stress were evaluated with the use of human blood. The level of reactive oxygen species (ROS) was measured by the chemiluminescence (CL) method to evaluate the oxidative stress. Furthermore, the removal of bacterial endotoxin (lipopolysaccharide) by CLA-loaded PAN-NlPAAm was measured with enzyme-linked immunosorbent assay. The results show that the LCST swelling curve,vas at 37 degrees C. PNIPAAm chains form expanded structures under 37 degrees C. At temperatures greater than 37 degrees C, however, die chains farm compact structures by dehydration. This makes PNIPAAM membrane due to rapid hydration and dehydration changes of the polymer chain. In addition, the swelling ratio increased by 71% when the pH increased from 5 to 10, The concentration of LPS can be reduced by CLA-loaded PAN-NIPAAm 2.1 and 1.2 times of that by unmodified PAN and PAN-NIPAAm membranes, respectively. In addition, the level of ROS against CLA-loaded PAN-NIPAAm was reduced significantly than that against unmodified PAN and PAN-NIPAAm. Therefore CLA-loaded PAN-NIPAAm membrane could offer protection for patients against oxidative stress and could also inhibit lipopolysaccharide for clinical applications.
机译：在水解聚丙烯腈（PAN）膜的表面上原位合成了一层薄的聚（N-异丙基丙烯酰胺）（pNIPAAm）。该薄层由于聚（丙烯酸）部分而表现出pH响应，并且由于pNIPAAm部分而表现出温度响应。在各种温度和pH下评估膜的溶胀行为。 PAN-NIPAAm的溶胀比曲线显示出较低的临界溶液温度。然后，将共轭亚油酸（CLA）装入pNIPAAm层。使用人体血液评估了CLA对凝血和氧化应激的影响。通过化学发光（CL）方法测量活性氧（ROS）的水平，以评估氧化应激。此外，用酶联免疫吸附测定法测量了通过负载CLA的PAN-NlPAAm对细菌内毒素（脂多糖）的去除。结果表明，LCST的溶胀曲线在37摄氏度时呈上升趋势。PNIPAAm链在37摄氏度时形成膨胀结构。然而，在高于37摄氏度的温度下，模链通过脱水形成紧密的结构。这使得PNIPAAM膜由于聚合物链的快速水合和脱水变化。此外，当pH从5增加到10时，溶胀率提高了71％。CLA负载的PAN-NIPAAm的LPS浓度可以降低2.1倍，而未改性PAN和PAN-NIPAAm膜的LPS浓度则可以降低1.2倍。另外，与未修饰的PAN和PAN-NIPAAm相比，抗CLA的PAN-NIPAAm的ROS水平显着降低。因此，加载CLA的PAN-NIPAAm膜可以为患者提供抗氧化应激的保护，并可以抑制脂多糖用于临床。

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