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首页> 外文期刊>Journal of Molecular Biology >PRION PROTEIN GENE VARIATION AMONG PRIMATES
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PRION PROTEIN GENE VARIATION AMONG PRIMATES

机译：原始蛋白中G蛋白基因的变异

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Prion diseases are manifest as genetic, sporadic or infectious neurodegenerative disorders in humans and animals. The prolonged incubation times that accompany the transmission of prions between species are due, at least in part, to differences in prion protein (PrP) sequence. To examine the species barriers between non-human primates and humans, we sequenced the open reading frames (ORF) of 25 PrP genes from apes and monkeys. Comparison of the PrP genes of these animals with that of humans showed amino acid identities ranging from 92.9 to 99.6%. While phylograms of primate PrP sequences revealed a novel branching pattern for the apes, the genomic organization of all the primate PrP genes was similar, with the entire ORF contained within a single exon. Alignment of variant residues in primates, rodents and domestic animals showed no concordance with the mutations that segregate with human prion diseases or with polymorphisms that modulate disease in humans, mice and sheep. Most substitutions were conservative and, characteristically, clustered outside the four putative alpha-helical regions that are thought to form a four-helix bundle in the cellular isoform of PrP (PrPC). Deletion of one of five Gly-Pro rich octarepeats from the N-terminus of PrP was seen in some species, while squirrel monkeys had an additional octarepeat; squirrel monkeys have been frequently used as experimental hosts for transmission of human prions. Alignment of primate and other mammalian PrP sequences suggests that codons between 90 and 130 have a profound influence on the transmissibility of prions from one species to another. [References: 99]
机译：on病毒是人类和动物的遗传性，散发性或感染性神经退行性疾病。 species病毒在物种之间传播的时间延长，至少部分是由于病毒蛋白（PrP）序列的差异。为了检查非人类灵长类动物与人类之间的物种屏障，我们对来自猿类和猴子的25个PrP基因的开放阅读框（ORF）进行了测序。将这些动物的PrP基因与人类的PrP基因进行比较，发现其氨基酸同一性范围为92.9至99.6％。虽然灵长类动物PrP序列的系统进化图揭示了猿类的新型分支模式，但所有灵长类动物PrP基因的基因组组织相似，整个ORF包含在单个外显子中。灵长类，啮齿动物和家畜中的变异残基比对显示与人类human病毒疾病或调节人类，小鼠和绵羊疾病的多态性分离的突变不一致。大多数取代是保守的，并且典型地聚集在四个推定的α-螺旋区域之外，这些区域被认为在PrP（PrPC）的细胞亚型中形成了四螺旋束。在某些物种中，从PrP的N末端删除了五种富含Gly-Pro的八角豆中的一种，而松鼠的猴子则有另一八角形。松鼠猴经常被用作人类hosts病毒传播的实验宿主。灵长类和其他哺乳动物PrP序列的比对表明，90至130之间的密码子对病毒从一种物种向另一种物种的传播具有深远的影响。 [参考：99]

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