• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >NMR solution structure and DNA-binding model of the DNA-binding domain of competence protein A.
【24h】

NMR solution structure and DNA-binding model of the DNA-binding domain of competence protein A.

机译：能力蛋白A的DNA结合域的NMR溶液结构和DNA结合模型。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Competence protein A (ComA) is a response regulator protein involved in the development of genetic competence in the Gram-positive spore-forming bacterium Bacillus subtilis, as well as the regulation of the production of degradative enzymes and antibiotic synthesis. ComA belongs to the NarL family of proteins, which are characterized by a C-terminal transcriptional activator domain that consists of a bundle of four helices, where the second and third helices (alpha 8 and alpha 9) form a helix-turn-helix DNA-binding domain. Using NMR spectroscopy, the high-resolution 3D solution structure of the C-terminal DNA-binding domain of ComA (ComAC) has been determined. In addition, surface plasmon resonance and NMR protein-DNA titration experiments allowed for the analysis of the interaction of ComAC with its target DNA sequences. Combining the solution structure and biochemical data, a model of ComAC bound to the ComA recognition sequences on the srfA promoter has been developed. The model shows that for DNA binding, ComA uses the conserved helix-turn-helix motif present in other NarL family members. However, the model reveals also that ComA might use a slightly different part of the helix-turn-helix motif and there appears to be some associated domain re-orientation. These observations suggest a basis for DNA binding specificity within the NarL family.
机译：能力蛋白A（ComA）是一种响应调节蛋白，参与革兰氏阳性孢子形成细菌枯草芽孢杆菌的遗传能力的发展，以及降解酶的产生和抗生素合成的调控。 ComA属于NarL蛋白质家族，其特征是C端转录激活结构域由四个螺旋束组成，其中第二个和第三个螺旋（alpha 8和alpha 9）形成螺旋-转-螺旋DNA绑定域。使用NMR光谱法，已经确定了ComA（ComAC）C末端DNA结合结构域的高分辨率3D溶液结构。此外，表面等离振子共振和NMR蛋白质-DNA滴定实验可以分析ComAC及其靶DNA序列的相互作用。结合溶液结构和生化数据，已经开发了与srfA启动子上的ComA识别序列结合的ComAC模型。该模型显示，对于DNA结合，ComA使用其他NarL家族成员中存在的保守的螺旋-转-螺旋基序。但是，该模型还表明，ComA可能使用了螺旋-转-螺旋基序的稍微不同的部分，并且似乎存在一些相关的域重新定向。这些发现为NarL家族内DNA结合特异性奠定了基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号