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Effects of salts on internal DNA pressure and mechanical properties of phage capsids.

机译:盐对内部DNA压力和噬菌体衣壳力学性能的影响。

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摘要

Based on atomic force microscopy nanoindentation measurements of phage lambda, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg(2+) and Sp(4+)). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage varphi29 and herpes simplex virus type 1 particles.
机译:基于噬菌体λ的原子力显微镜纳米压痕测量,我们先前提出了一个最小模型,该模型描述了水合DNA的作用,该作用可增强病毒衣壳抵抗野生型DNA堆积密度时的外部变形。在这里,我们通过测试以下预测来报告该模型的证据:通过添加多价离子(Mg(2+)和Sp(4+))可以大大降低DNA水合力。使用没有可调整参数的DNA水化模型解释了这些结果。该模型还预测了其他充满DNA的衣壳的刚度,我们使用噬菌体varphi29和1型单纯疱疹病毒证实了这一点。

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