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首页> 外文期刊>Journal of Molecular Biology >Cytoskeletal Control of Nuclear Morphology and Chromatin Organization
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Cytoskeletal Control of Nuclear Morphology and Chromatin Organization

机译：核形态和染色质组织的细胞骨架控制。

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摘要

The nucleus is sculpted toward various morphologies during cellular differentiation and development. Alterations in nuclear shape often result in changes to chromatin organization and genome function. This is thought to be reflective of its role as a cellular mechanotransducer. Recent evidence has highlighted the importance of cytoskeletal organization in defining how nuclear morphology regulates chromatin dynamics. However, the mechanisms underlying cytoskeletal control of chromatin remodeling are not well understood. We demonstrate here the differential influence of perinuclear actin- and microtubule-driven assemblies on nuclear architecture using pharmacological inhibitors and targeted RNA interference knockdown of cytoskeleton components in Drosophila cells. We find evidence that the loss of perinuclear actin assembly results in basolateral enhancement of microtubule organization and this is reflected functionally by enhanced nuclear dynamics. Cytoskeleton reorganization leads to nuclear lamina deformation that influences heterochromatin localization and core histone protein mobility. We also show that modulations in actin microtubule assembly result in differential gene expression patterns. Taken together, we suggest that perinuclear actin and basolateral microtubule organization exerts mechanical control on nuclear morphology and chromatin dynamics. (c) 2014 Elsevier Ltd. All rights reserved.
机译：在细胞分化和发育过程中，细胞核被雕刻成各种形态。核形状的改变通常会导致染色质组织和基因组功能的改变。认为这反映了其作为细胞机械换能器的作用。最近的证据强调了在确定核形态如何调节染色质动力学中细胞骨架组织的重要性。但是，尚不完全了解染色质重塑的细胞骨架控制的机制。我们在这里证明了周周肌动蛋白和微管驱动的程序集对使用药理学抑制剂和果蝇细胞中细胞骨架成分的靶向RNA干扰敲除对核结构的影响。我们发现证据表明，核周肌动蛋白装配的丧失导致微管组织的基底外侧增强，这在功能上通过增强的核动力学反映出来。细胞骨架重组导致核层薄板变形，影响异染色质的定位和核心组蛋白的移动性。我们还显示肌动蛋白微管组装中的调制导致差异基因表达模式。两者合计，我们建议核周肌动蛋白和基底外侧微管组织对核形态和染色质动力学施加机械控制。 （c）2014 Elsevier Ltd.保留所有权利。

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