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Deciphering Complexity in Molecular Biophysics with Single-Molecule Resolution

机译:用单分子分辨率解​​析分子生物物理学中的复杂性

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The structural features and dynamics of biological macromolecules underlie the molecular biology and correct functioning of cells. However, heterogeneity and other complexity of these molecules and their interactions often lead to loss of important information in traditional biophysical experiments. Single-molecule methods have dramatically altered the conceptual thinking and experimental tests available for such studies, leveraging their ability to avoid ensemble averaging. Here, I discuss briefly the rise of fluorescence single-molecule methods over the past two decades, a few key applications, and end with a view to challenges and future prospects. (C) 2015 Elsevier Ltd. All rights reserved.
机译:生物大分子的结构特征和动力学是分子生物学和细胞正确功能的基础。但是,这些分子的异质性和其他复杂性以及它们之间的相互作用通常会导致传统生物物理实验中重要信息的丢失。单分子方法充分利用了避免整体平均的能力,极大地改变了此类研究的概念思维和实验测试。在这里,我简要地讨论了荧光单分子方法在过去二十年中的兴起,一些关键应用,并以挑战和未来前景为结尾。 (C)2015 Elsevier Ltd.保留所有权利。

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