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首页> 外文期刊>Journal of Molecular Biology >A PII-Like Protein Regulated by Bicarbonate: Structural and Biochemical Studies of the Carboxysome-Associated CPII Protein
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A PII-Like Protein Regulated by Bicarbonate: Structural and Biochemical Studies of the Carboxysome-Associated CPII Protein

机译：由碳酸氢盐调节的PII样蛋白质：与羧基相关的CPII蛋白质的结构和生化研究

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Autotrophic bacteria rely on various mechanisms to increase intracellular concentrations of inorganic forms of carbon (i.e., bicarbonate and CO2) in order to improve the efficiency with which they can be converted to organic forms. Transmembrane bicarbonate transporters and carboxysomes play key roles in accumulating bicarbonate and CO2, but other regulatory elements of carbon concentration mechanisms in bacteria are less understood. In this study, after analyzing the genomic regions around a-type carboxysome operons, we characterize a protein that is conserved across these operons but has not been previously studied. On the basis of a series of apo- and ligand-bound crystal structures and supporting biochemical data, we show that this protein, which we refer to as the carboxysome-associated PII protein (CPII), represents a new and distinct subfamily within the broad superfamily of previously studied PII regulatory proteins, which are generally involved in regulating nitrogen metabolism in bacteria. CPII undergoes dramatic conformational changes in response to ADP binding, and the affinity for nucleotide binding is strongly enhanced by the presence of bicarbonate. CPII therefore appears to be a unique type of PII protein that senses bicarbonate availability, consistent with its apparent genomic association with the carboxysome and its constituents. (C) 2016 Elsevier Ltd. All rights reserved.
机译：自养细菌依靠多种机制来增加细胞内无机形式的碳（即碳酸氢盐和二氧化碳）的浓度，以提高其转化为有机形式的效率。跨膜碳酸氢盐转运蛋白和羧基体在积累碳酸氢盐和二氧化碳中起着关键作用，但是细菌中碳浓度机制的其他调控元素却鲜为人知。在这项研究中，在分析了a型羧基体操纵子周围的基因组区域之后，我们表征了在这些操纵子中保守但尚未进行过研究的蛋白质。根据一系列载脂蛋白和配体结合的晶体结构以及支持的生化数据，我们表明该蛋白（我们称为与羧基体相关的PII蛋白（CPII））代表了广泛的新的亚家族以前研究过的PII调节蛋白的超家族，通常参与调节细菌中氮的代谢。 CPII响应ADP结合而发生剧烈的构象变化，并且碳酸氢盐的存在大大增强了核苷酸结合的亲和力。因此，CPII似乎是一种独特的PII蛋白，可感知碳酸氢盐的可利用性，这与其与羧基体及其成分的表观基因组关联是一致的。 （C）2016 Elsevier Ltd.保留所有权利。

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