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首页> 外文期刊>Journal of Physics. Condensed Matter >spFRET reveals changes in nucleosome breathing by neighboring nucleosomes
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spFRET reveals changes in nucleosome breathing by neighboring nucleosomes

机译：spFRET显示邻近核小体的核小体呼吸变化

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摘要

Chromatin, the structure in which DNA is compacted in eukaryotic cells, plays a key role in regulating DNA accessibility. FRET experiments on single nucleosomes, the basic units in chromatin, have revealed a dynamic nucleosome where spontaneous DNA unwrapping from the ends provides access to the nucleosomal DNA. Here we investigated how this DNA breathing is affected by extension of the linker DNA and by the presence of a neighboring nucleosome. We found that both electrostatic interactions between the entering and exiting linker DNA and nucleosome-nucleosome interactions increase unwrapping. Interactions between neighboring nucleosomes are more likely in dinucleosomes spaced by 55 bp of linker DNA than in dinucleosomes spaced by 50 bp of linker DNA. Such increased unwrapping may not only increase the accessibility of nucleosomal DNA in chromatin fibers, it may also be key to folding of nucleosomes into higher order structures.
机译：染色质是在真核细胞中压紧DNA的结构，在调节DNA的可及性中起关键作用。在单个核小体（染色质中的基本单位）上进行的FRET实验揭示了一种动态的核小体，从末端解开的自发DNA可提供对核小体DNA的访问。在这里，我们研究了这种DNA呼吸如何受到接头DNA的延伸和邻近核小体的存在的影响。我们发现进入和退出的连接子DNA之间的静电相互作用和核小体-核小体之间的相互作用都增加了解缠。间隔55 bp的接头DNA的双核小体中，比间隔50 bp的接头DNA的双核小体中的相邻核小体之间的相互作用更有可能。这种增加的解缠不仅可能增加染色质纤维中核小体DNA的可及性，也可能是将核小体折叠成更高阶结构的关键。

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