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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Antilisterial and physical properties of polysaccharide-collagen films embedded with cell-free supernatant of Lactococcus lactis
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Antilisterial and physical properties of polysaccharide-collagen films embedded with cell-free supernatant of Lactococcus lactis

机译:嵌入细胞上清液乳酸乳乳乳乳糖乳糖膜的止血和物理性质

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The physical and antimicrobial properties of sodium alginate or sodium carboxymethylcellulose films containing cell-free supernatant of Lactococcus lactis ATCC 11454 were evaluated in this study. The antimicrobial activity of the films against Staphylococcus aureus ATCC 6538, and Escherichia coli ATCC 25922 was evaluated. Cell-free supernatants were obtained by centrifugation and filtration of Lactococcus lactis cultures in MRS broth; the filtrates were freeze-dried, and rehydrated (4, 8, or 12 mg/mL) to produce collagen film. The thickness, tensile strength, color, moisture content, solubility, and water vapor permeability of the films were measured to examine the effects of cell-free supernatants on film matrices. Noticeable antimicrobial activity was observed against Staphylococcus aureus, and Escherichia coli upon the addition of 12 mg/mL cell-free supernatants. Our results indicate that the incorporation of cell-free supernatant changed the physical properties of the edible films. Gradual and significant (p < 0.05) increase in color difference and solubility were observed with the addition of increasing concentrations of cell-free supernatant. Antimicrobial films were brown in color and with variable moisture contents. Sodium alginate films were better than sodium carboxymethylcellulose films for most of the evaluated parameters. In summary, collagen films containing cell-free supernatants are interesting alternative natural antimicrobial films for functional food packaging. (C) 2019 Elsevier B.V. All rights reserved.
机译:在本研究中评估了含藻酸钠或羧甲基纤维素膜的物理和抗微生物性能含有无细胞上清液11454的无细胞上清液。评价逆葡萄球菌ATCC 6538和大肠杆菌ATCC 25922对粘附膜的抗微生物活性。通过在肉汤中的乳晕乳酸乳乳乳乳乳乳杆菌培养物中离心和过滤来获得无细胞上清液;将滤液冷冻干燥,并再水化(4,8或12mg / ml)以产生胶原膜。测量薄膜的厚度,拉伸强度,颜色,水分含量,溶解度和水蒸气渗透性,以检查无细胞上清液对膜基质的影响。在加入12mg / ml无细胞上清液时观察到葡萄球菌和大肠杆菌的显着抗微生物活性。我们的结果表明,无细胞上清液的掺入改变了可食用薄膜的物理性质。通过添加增加的无细胞上清液,观察到色差和溶解度的逐渐和显着(P <0.05)。抗微生物膜的颜色棕色和可变性水分。对于大多数评估参数,藻酸钠薄膜优于羧甲基纤维素薄膜。总之,含有无细胞上清液的胶原蛋白是有趣的用于功能性食品包装的替代天然抗微生物薄膜。 (c)2019 Elsevier B.v.保留所有权利。

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