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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Immunomodulating activity of the polysaccharide TLH-3 from Tricholomalobayense in RAW264.7 macrophages
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Immunomodulating activity of the polysaccharide TLH-3 from Tricholomalobayense in RAW264.7 macrophages

机译：来自Raw264.7巨噬细胞的Tricholomalobayense的多糖TLH-3的免疫调节活性

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摘要

Polysaccharide TLH-3 extracted from Tricholoma lobayense possessed unique antioxidant and anti-aging activities, whereas its immunomodulatory properties remain unexplored. Herein in order to explore TLH-3 biological activities, the immunomodulatory effects on RAW264.7 macrophages and its molecular mechanisms were investigated. It was showed that TLH-3 could significantly enhance the phagocytic activity, releasing toxic molecules NO (nitricoxide), secretion of the cytokine TNF-?(tumor necrosis factor-?, IL-6 (interleukin-6). Further, TNF-?and IL-6 were blocked by the inhibitor of TLR4 (Toll-like receptor4), suggesting TLR4 was a receptor of TLH-3, and immunomodulatory activity of TLH-3 was mediated by TLR4. Moreover, immunofluorescence indicated that TLH-3 lead to the nuclear translocation of NF-?B (nuclear factor-?B) subunit p65. Western blotting demonstrated that NF-?B levels in nucleuses increased and cytoplasmic I?B-?(inhibitor of NF-?B) degraded after TLH-3 treatment, suggesting that TLH-3 probably stimulated macrophage by activating the IKB-alpha-NF-kappa B pathway via TLR-4. This study demonstrated that TLH-3 could be potentially used as immunomodulatory agent for healthcare and disease control. (C) 2017 Elsevier B.V. All rights reserved.
机译：从幼状robayense中提取的多糖TLH-3具有独特的抗氧化和抗衰老活动，而其免疫调节性能仍然是未开发的。在此为了探索TLH-3生物活性，研究了对Raw264.7巨噬细胞的免疫调节作用及其分子机制。结果表明，TLH-3可以显着增强吞噬活性，释放有毒分子（硝基氧化氢），细胞因子TNF的分泌 - ？（肿瘤坏死因子 - α，IL-6（白细胞介素-6）。进一步，TNF-？通过TLR4的抑制剂（Toll样受体4）阻断IL-6，表明TLR4是TLH-3的受体，TLH-3的免疫调节活性由TLR4介导。此外，免疫荧光表明TLH-3导致NF-αb（核因子-αb）亚单位p65的核易位。Western印迹表明，核中的NF-αb水平增加和细胞质I≤βb - β（NF-Δb的抑制剂）在TLH-3后降解治疗，表明TLH-3通过TLR-4激活IKB-α-NF-Kappa B途径可能刺激巨噬细胞。该研究表明，TLH-3可能被潜在用作医疗保健和疾病控制的免疫调节剂。（c） 2017年Elsevier BV保留所有权利。

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