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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Chitosans as new tools against biofilms formation on the surface of silicone urinary catheters
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Chitosans as new tools against biofilms formation on the surface of silicone urinary catheters

机译：壳聚糖作为对硅胶尿管表面上的生物膜形成的新工具

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Urinary catheters contamination by microorganisms is a major cause of hospital acquired infections and represents a limitation for long-term use. In this work, biofilms of Klebsiella pneumoniae and Escherichia coli clinical isolates were developed on urinary catheters for 48 and 72 h in artificial urine medium (AUM) with different molecular weight chitosans (AUM-CS solutions) at pH 5.0. The number of viable bacteria was determined by standard plate count agar while crystal violet (CV) staining was carried out to assess biomass production (optical density at 570 nm) in the mentioned conditions. Re-growth of each strain was also evaluated after 24 h re incubation of the treated catheters. Significant decreases of log CFU/catheter and biomass production were observed for all the biofilms developed in AUM-CS compared with the controls in AUM. The percentages of biofilm removal were slightly higher for E. coil biofilms (up to 90.4%) than those of K. pneumoniae (89.7%); in most cases, the complete inhibition of bacterial re-growth on treated catheter pieces was observed. Contact time influenced chitosan efficacy rather than its molecular weight or the biofilms age. The results confirmed the potentiality of chitosans as a biomacromolecule tool to contrast biofilm formation and reduce bacterial re-growth on urinary catheters. (C) 2018 Elsevier B.V. All rights reserved.
机译：微生物污染的尿导管是医院获得的感染的主要原因，并且代表了长期使用的限制。在这项工作中，在人工尿介质（Aum）中，在pH 5.0的不同分子量壳聚糖（Aum-CS溶液）中，在尿导管中开发了Klebsiella肺炎和大肠杆菌临床分离物的生物膜。通过标准板计数琼脂测定活细菌的数量，而晶体紫（CV）染色是在提到的条件下评估生物质产生（在570nm处的光密度）。在经处理​​的导管孵育后，还评估每种菌株的重新生长。与Aum中的对照相比，观察到在Aum-CS中产生的所有生物膜的对数CFU /导管和生物量产生的显着降低。 E.卷曲生物膜（高达90.4％）的生物膜去除百分比略高于K.肺炎（89.7％）;在大多数情况下，观察到在处理过的导管片上完全抑制细菌重新生长。接触时间影响壳聚糖功效而不是其分子量或生物膜年龄。结果证实壳聚糖作为生物致抗体的潜力，以对比生物膜形成并降低尿导管上的细菌重新生长。 （c）2018年elestvier b.v.保留所有权利。

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