• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >A facile molecularly imprinted polymer-based fluorometric assay for detection of histamine
【24h】

A facile molecularly imprinted polymer-based fluorometric assay for detection of histamine

机译：用于检测组胺的容易分子印迹聚合物基荧光测定法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, o-phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 mM with the limit of detection of 1.80 mM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85%.
机译：组胺是在许多身体细胞中自然存在的生物胺。它也是一种污染物，主要在宠坏的食物中发现。含有高水平组胺的食物的消耗可能导致过敏的食物中毒。因此，可以迫切需要常规筛选组胺的分析方法。在本文中，我们开发了一种容易和成本效益的分子印迹聚合物（MIP）基础的荧光测定以直接量化组胺。使用改性的固相合成方法合成组胺特异性MIP纳米颗粒（NanoMIPS）。然后将它们固定在微孔板的孔中以在水性样品中结合组胺。结合后，使用O-邻苯二甲醛（OPA）标记结合的组胺，将结合事件转化为荧光信号。所获得的组胺的校准曲线显示线性相关性范围为1.80至44.98 mm，检测限为1.80mm。该方法成功地用于检测尖刺日记牛奶中的组胺，回收率超过85％。

著录项

  • 来源
    《RSC Advances》 |2018年第5期|共8页
  • 作者

    Feng Xiaotong; Ashley Jon; Zhou Tongchang; Halder Arnab; Sun Yi;

  • 作者单位

    Tech Univ Denmark Dept Micro &

    Nanotechnol DK-2800 Lyngby Denmark;

    Tech Univ Denmark Dept Micro &

    Nanotechnol DK-2800 Lyngby Denmark;

    Tech Univ Denmark Dept Micro &

    Nanotechnol DK-2800 Lyngby Denmark;

    Tech Univ Denmark Dept Micro &

    Nanotechnol DK-2800 Lyngby Denmark;

    Tech Univ Denmark Dept Micro &

    Nanotechnol DK-2800 Lyngby Denmark;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号