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'Turn on' room-temperature phosphorescent biosensors for detection of hyaluronic acid based on manganese-doped ZnS quantum dots

机译:“打开”室温磷光生物传感器,用于检测锰掺杂ZnS量子点的透明质酸

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摘要

Biosensors based on excellent optical properties of quantum dots (QDs) nanohybrids are efficient for biological detection. In this work, a room-temperature phosphorescent (RTP) PDAD-Mn-ZnS QDs biosensor was constructed with poly( diallyldimethylammonium chloride) (PDAD) as the modifier of MPA-capped Mn-ZnS QDs, and used to detect hyaluronic acid (HA). The newly-added HA induced severe electrostatic interaction with PDAD-Mn-ZnS QDs, leading to the aggregation between PDAD-Mn-ZnS QDs and HA and thereby enhancing RTP. The enhancement of RTP was proportional to the HA concentrations within certain ranges. On this basis, a high-performance HA sensor was built and this sensor had a detection limit of 0.03 mu g mL(-1) and a detection range of 0.08-2.8 mu g mL(-1). This proposed RTP sensor can avoid interferences from the background fluorescence or scattering light of the matrix that are encountered in spectrofluorometry. Thus, this biosensor is potentially suitable for detection of HA in real samples without complicated pretreatment.
机译:基于量子点(QDS)纳米油的优异光学性能的生物传感器是有效的生物检测。在这项工作中,使用聚(二烯丙基甲基氯化铵)(PDAD)作为MPA封端的MN-ZnS QDS的改性剂构建了室温磷光(RTP)Pdad-Mn-ZnS QDS生物传感器,并用于检测透明质酸(HA )。新增的HA诱导与PDAD-MN-ZNS QDS的严重静电相互作用,导致PDAD-MN-ZNS QD和HA之间的聚集,从而增强RTP。 RTP的增强与某些范围内的HA浓度成比例。在此基础上,构建了高性能HA传感器,该传感器的检出限为0.03μg(-1),检测范围为0.08-2.8μgml(-1)。该提出的RTP传感器可以避免从光谱荧光测定中遇到的基质的背景荧光或散射光的干扰。因此,这种生物传感器可能适用于在实际样品中检测HA而无需复杂预处理。

著录项

  • 来源
    《RSC Advances》 |2018年第6期|共7页
  • 作者单位

    Shanxi Normal Univ Linfen 041004 Peoples R China;

    Shanxi Normal Univ Linfen 041004 Peoples R China;

    Shanxi Normal Univ Linfen 041004 Peoples R China;

    Shanxi Normal Univ Linfen 041004 Peoples R China;

    Shanxi Normal Univ Linfen 041004 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

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