• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Biochemical and kinetic characterization of laccase and manganese peroxidase from novel Klebsiella pneumoniae strains and their application in bioethanol production
【24h】

Biochemical and kinetic characterization of laccase and manganese peroxidase from novel Klebsiella pneumoniae strains and their application in bioethanol production

机译：新型猕猴桃菌株菌株曲碱和锰过氧化物酶的生化和动力学酶及其在生物乙醇生产中的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Laccase (lac) and manganese peroxidase (MnP) enzymes from the novel Klebsiella pneumoniae isolates, grown on lignin basic media (LBM) were purified by 80% ammonium sulphate fractionation, dialysis and DEAE-sepharose column chromatography. The optimum temperatures for laccase production were 60 degrees C, 50 degrees C and 50 degrees C and for MnP production were 50 degrees C, 70 degrees C and 60 degrees C from NITW715076_2, NITW715076_1 and NITW715076 isolates, respectively. The optimal pH for production was found to be 5 for production of both the enzymes from all the isolates. 2.8-3.5 fold enzyme purification was achieved retaining around 60-70% of the initial activity. SDS-PAGE revealed the molecular mass of laccase and MnP to be 66 kDa and 48 kDa, respectively. The substrate ABTS and MnSO4 exhibited more specificity towards NITW715075_2 derived laccase and MnP (lac: K-m = 0.38 mM, V-max = 71.42 U ml(-1); MnP: K-m = 0.17 mM, V-max = 106.38 U ml(-1)) compared to NITW715076_1 (lac: K-m = 3.97 mM, V-max = 148.8 U ml(-1); MnP: K-m = 0.90 mM, V-max = 114.67 U ml(-1)) and NITW715076 (lac: K-m = 0.46 mM, V-max = 23.42 U ml(-1); MnP: K-m = 0.19 mM, V-max = 108.10 U ml(-1)) derived. l-Cysteine and sodium azide imposed a strong inhibitory effect on the activities of both the enzymes. EDTA inhibited laccase and MnP activity at higher concentration. SDS strongly inhibited activity while for MnP it showed less inhibitory effect. The enzymes were employed for ethanol production from rice and wheat bran biomass which showed 39.29% improved production compared to control. After evaluating the applicability of these enzymes it can be suggested that the ligninolytic enzyme of Klebsiella pneumoniae isolates could be effectively employed in enhanced ethanol production and could be explored for other putative applications.
机译：从新的Klebsiella肺炎群分离株中生长的漆酶（LAC）和锰过氧化物酶（MNP）酶，通过80％硫酸铵分级，透析和DEA-Sepharose柱色谱法纯化于木质素碱性培养基（LBM）上生长。漆酶生产的最佳温度为60℃，50℃和50℃，MNP产生分别为50℃，70℃和60摄氏度，分别为Nitw715076_2，Nitw715076_1和Nitw715076分离株。发现生产的最佳pH值为5，用于生产来自所有分离株的酶。 2.8-3.5折叠酶纯化，达到初始活性的约60-70％。 SDS-PAGE揭示了漆酶和MNP的分子量，分别为66kDa和48kDa。底物ABT和MNSO4对NITW715075_2衍生的漆酶和MNP（LAC：Km = 0.38mm，V-MAX = 71.42umL（-1）; MNP：KM = 0.17mm，V-MAX = 106.38 U mL（ - 1））与NITW715076_1（LAC：KM = 3.97mm，V-MAX = 148.8 U mL（-1）; MNP：KM = 0.90 mm，V-MAX = 114.67 U mL（-1））和NITW715076（LAC： km = 0.46 mm，V-max = 23.42 u ml（-1）; mnp：km = 0.19 mm，v-max = 108.10 u ml（-1））衍生。 L-半胱氨酸和叠氮化钠对两种酶的活性施加了强烈的抑制作用。 EDTA在较高浓度下抑制漆酶和MNP活性。 SDS强烈抑制活性，而MNP表现出较少的抑制作用。酶用于从水稻和小麦麸皮的乙醇生产，其显示与对照相比，增加了39.29％的改进的生产。在评估这些酶的适用性之后，可以提出Klebsiella肺炎群岛的木质素溶解酶可以有效地用于增强乙醇生产中，可以探索其他推定应用。

著录项

  • 来源
    《RSC Advances》 |2018年第27期|共12页
  • 作者

    Gaur Nisha; Narasimhulu Korrapati; Pydisetty Y.;

  • 作者单位

    Natl Inst Technol Warangal Dept Biotechnol Warangal 506004 Telangana India;

    Natl Inst Technol Warangal Dept Biotechnol Warangal 506004 Telangana India;

    Natl Inst Technol Warangal Dept Chem Engn Warangal 506004 Telangana India;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号