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Screening of bacteria-binding peptides and one-pot ZnO surface modification for bacterial cell entrapment

机译:细菌细胞抑制细菌结合肽和单壶ZnO表面改性的筛选

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摘要

Short functional peptides are promising materials for use as targeting recognition probes. Toll-like receptor 4 (TLR4) plays an essential role in pathogen recognition and in activation of innate immunity. Here, the TLR4 amino acid sequence was used to screen for bacterial cell binding peptides using a peptide array. Several octamer peptides, including GRHIFWRR, demonstrated binding to Escherichia coli as well as lipopolysaccharides. Linking this peptide with the ZnO-binding peptide HKVAPR, creates a bi-functional peptide capable of one-step ZnO surface modification for bacterial cell entrapment. Ten-fold increase in entrapment of E. coli was observed using the bi-functional peptide. The screened peptides and the simple strategy for nanomaterial surface functionalization can be employed for various biotechnological applications including bacterial cell entrapment onto ZnO surfaces.
机译:短功能肽是有希望的材料,用于靶向识别探针。 Toll样受体4(TLR4)在病原体识别和先天免疫激活中起重要作用。 这里,使用肽阵列使用TLR4氨基酸序列用于筛选细菌细胞结合肽。 包括GRHIFWRR在内的几种八寡肽,表现出与大肠杆菌和脂多糖的结合。 将该肽与ZnO结合肽HKVAPR连接,产生能够进行一步ZnO表面改性的双官能肽,用于细菌细胞夹带。 使用双官能肽观察到大肠杆菌夹杂物增加的十倍。 筛选的肽和纳米材料官能化的简单策略可用于各种生物技术应用,包括细菌细胞夹在ZnO表面上。

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  • 来源
    《RSC Advances》 |2018年第16期|共5页
  • 作者单位

    Tokyo Inst Technol Dept Chem Sci &

    Engn Sch Mat &

    Chem Technol Meguro Ku 2-12-1 O Okayama Tokyo 1528552 Japan;

    Tokyo Inst Technol Dept Chem Sci &

    Engn Sch Mat &

    Chem Technol Meguro Ku 2-12-1 O Okayama Tokyo 1528552 Japan;

    Tokyo Inst Technol Dept Chem Sci &

    Engn Sch Mat &

    Chem Technol Meguro Ku 2-12-1 O Okayama Tokyo 1528552 Japan;

    Tokyo Inst Technol Dept Chem Sci &

    Engn Sch Mat &

    Chem Technol Meguro Ku 2-12-1 O Okayama Tokyo 1528552 Japan;

    Tokyo Inst Technol Dept Chem Sci &

    Engn Sch Mat &

    Chem Technol Meguro Ku 2-12-1 O Okayama Tokyo 1528552 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

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