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Efficient production of high-molecular-weight hyaluronic acid with a two-stage fermentation

机译：高分子重量透明质酸的高分性透明质酸，两阶段发酵

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摘要

In this paper, the production pattern of hyaluronic acid (HA) was revealed: the chain growth of HA mainly occurred in the first half of fermentation while the product accumulated throughout the fermentation period. Attempts were made to develop a two-stage fermentation process which provided high-level synthesis in both product titer and molecular weight. The pH was kept at 8.0 with a temperature of 31 degrees C in the first fermentation stage (0-10 h) to promote the growth of weight-average molecular weight (M-w), and the pH and temperature were maintained at 7.0 and 37 degrees C, respectively, in the following fermentation stage to facilitate HA accumulation. In addition, constant rates of aeration (1 vvm) and agitation (600 rpm) were adopted. The two-stage fermentation provided a balanced result in which a product titer of 4.75 g L-1 and a M-w of 2.36 x 10(6) Da were achieved under optimized conditions. The process introduces an effective way to produce HA considering the effect of segmented control strategy.
机译：在本文中，揭示了透明质酸（HA）的生产模式：HA的链生长主要发生在发酵的前半部分，同时在整个发酵期间积累。尝试开发出一种两级发酵过程，其在产品滴度和分子量中提供高水平的合成。将pH保持在8.0与31摄氏度在第一发酵阶段（0-10 1H）的温度下，以促进重均分子量（Mw）的生长，并且pH和温度分别保持在7.0和37度C，分别在下面的发酵阶段，以便HA积累。此外，采用恒定曝气（1 VVM）和搅拌率（600 rpm）。两级发酵提供了平衡的结果，其中在优化条件下实现了4.75g L-1的产物滴度和2.36×10（6）达的M-W.该过程介绍了考虑分段控制策略的效果的生成HA的有效方法。

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  • 来源
    《RSC Advances》 |2018年第63期|共5页
  • 作者

    Liu J.; Wang Y.; Li Z.; Ren Y.; Zhao Y.; Zhao G.;

  • 作者单位

    Hebei Univ Sci &

    Technol Coll Biosci &

    Bioengn Fermentat Engn Technol Res Ctr Heibei Prov 26 Yuxiang Rd Shijiazhuang 050000 Hebei Peoples R China;

    Hebei Univ Sci &

    Technol Coll Biosci &

    Bioengn Fermentat Engn Technol Res Ctr Heibei Prov 26 Yuxiang Rd Shijiazhuang 050000 Hebei Peoples R China;

    Hebei Univ Sci &

    Technol Coll Biosci &

    Bioengn Fermentat Engn Technol Res Ctr Heibei Prov 26 Yuxiang Rd Shijiazhuang 050000 Hebei Peoples R China;

    Hebei Univ Sci &

    Technol Coll Biosci &

    Bioengn Fermentat Engn Technol Res Ctr Heibei Prov 26 Yuxiang Rd Shijiazhuang 050000 Hebei Peoples R China;

    Hebei Univ Sci &

    Technol Coll Biosci &

    Bioengn Fermentat Engn Technol Res Ctr Heibei Prov 26 Yuxiang Rd Shijiazhuang 050000 Hebei Peoples R China;

    Hebei Univ Sci &

    Technol Coll Biosci &

    Bioengn Fermentat Engn Technol Res Ctr Heibei Prov 26 Yuxiang Rd Shijiazhuang 050000 Hebei Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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