Biomaterials from human bone - probing organic fraction removal by chemical and enzymatic methods

Mamede A. P.; Vassalo A. R.; Cunha E.; Goncalves D.; Parker S. F.; Batista de Carvalho L. A. E.; Marques M. P. M.

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Biomaterials from human bone - probing organic fraction removal by chemical and enzymatic methods

机译：来自人骨的生物材料 - 化学和酶法探测有机分数除去

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Two different deproteination and defatting processes of human bone were investigated, by combined infrared and neutron techniques: a previously reported hydrazine extraction and a newly developed multi-enzymatic treatment. Complementary Fourier transform infrared total attenuated reflectance and inelastic neutron scattering spectroscopies were applied, allowing access to all vibrational modes of the samples. The effectiveness of the different experimental protocols for removing the organic constituents of bone (lipids and protein) was probed, as well as their effect on bone's structural and crystallinity features. The results thus gathered are expected to have an impact on bioanthropological, archaeological and medical sciences, namely regarding the development of novel biocompatible materials for orthopaedic xenografts.

机译：通过组合红外和中子技术研究了两种不同的脱核和脱离人骨的过程：先前报道的肼提取和新开发的多酶处理。互补傅里叶变换红外线总衰减反射率和无弹性中子散射光谱，允许进入样品的所有振动模式。探测了除去骨（脂质和蛋白质）的有机成分的不同实验方案的有效性，以及它们对骨骼结构和结晶度特征的影响。因此收集的结果预计对生物体学，考古和医学科学产生影响，即关于骨科异种移植物的新型生物相容性材料的发展。

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《RSC Advances》 |2018年第48期|共8页
作者
Mamede A. P.; Vassalo A. R.; Cunha E.; Goncalves D.; Parker S. F.; Batista de Carvalho L. A. E.; Marques M. P. M.;
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作者单位

Univ Coimbra Dept Chem Unidade I&

D Quim Fis Mol Coimbra Portugal;

Univ Coimbra Dept Chem Unidade I&

D Quim Fis Mol Coimbra Portugal;

Univ Coimbra Ctr Funct Ecol Lab Forens Anthropol Coimbra Portugal;

Univ Coimbra Ctr Funct Ecol Lab Forens Anthropol Coimbra Portugal;

STFC Rutherford Appleton Lab ISIS Facil Didcot OX11 0QX Oxon England;

Univ Coimbra Dept Chem Unidade I&

D Quim Fis Mol Coimbra Portugal;

Univ Coimbra Dept Chem Unidade I&

D Quim Fis Mol Coimbra Portugal;

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正文语种 eng
中图分类化学;
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1. Biomaterials from human bone - probing organic fraction removal by chemical and enzymatic methods [J] . Mamede A. P., Vassalo A. R., Cunha E., RSC Advances . 2018,第48期

机译：来自人骨的生物材料 - 化学和酶法探测有机分数除去
2. Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods [J] . A. P. Mamede, A. R. Vassalo, E. Cunha, RSC Advances . 2018,第48期

机译：人体骨骼中的生物材料-通过化学和酶促方法探测有机物去除
3. Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods [J] . A. P. Mamede, A. R. Vassalo, E. Cunha, RSC Advances . 2018,第48期

机译：人体骨骼中的生物材料-通过化学和酶促方法探测有机物去除
4. Chemically active organically doped sol-gel materials: enzymatic sensors chemical sensors and photoactive materials [C] . David Avnir, Hebrew Univ. of Jerusalem, Jerusalem, Conference on sol-gel optics . 1992

机译：化学活性有机掺杂溶胶-凝胶材料：酶传感器，化学传感器和光敏材料
5. Aspects of chemical, thermal, and enzymatic treatment, moisture content, and genetics on ruminal digestion and utilization of structural carbohydrate fractions in grass seed straw, and of non-structural carbohydrate fractions in beef finishing diets. [D] . Szasz, Josh I. 2004

机译：化学，热和酶处理，水分含量和遗传方面的反刍动物消化和利用草种子秸秆中的结构性碳水化合物组分以及牛肉精加工饮食中的非结构性碳水化合物组分。
6. Impact of the Different Preparation Methods to Obtain Human Adipose-Derived Stromal Vascular Fraction Cells (AD-SVFs) and Human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs): Enzymatic Digestion Versus Mechanical Centrifugation [O] . Pietro Gentile, Claudio Calabrese, Barbara De Angelis, 2019

机译：不同制备方法对人脂肪来源的间质干细胞（AD-SVF）和人脂肪来源的间充质干细胞（AD-MSC）的影响：酶消化与机械离心
7. Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods [O] . A. P. Mamede, A. R. Vassalo, E. Cunha, 2018

机译：来自人骨的生物材料 - 化学和酶法探测有机分数除去

Biomaterials from human bone - probing organic fraction removal by chemical and enzymatic methods

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