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首页> 外文期刊>RSC Advances >Molecular species fingerprinting and quantitative analysis of saffron (Crocus sativus L.) for quality control by MALDI mass spectrometry
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Molecular species fingerprinting and quantitative analysis of saffron (Crocus sativus L.) for quality control by MALDI mass spectrometry

机译：MARDI质谱法测定藏红花（CAROCUS SATIVUS L.）的分子物种指纹识别和定量分析

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摘要

Herein we describe a rapid, simple, and reliable method for the quantitative analysis and molecular species fingerprinting of saffron (Crocus sativus L.) by direct MS and MS/MS analysis. Experimentally, powdered saffron was subjected to a brief treatment with a 0.3% TFA water/acetonitrile solution, and the resulting mixture was directly placed on the MALDI plate for analysis. This approach allowed the detection of the commonly observed crocins C-1-C-6 and flavonols, together with the identification of the unknown highly glycosylated crocins C-7, C-8 and C-9, and carotenoid-derived metabolites. The strategy endorsed the simultaneous detection and characterization of saffron and adulterant markers using crude extracts of the adulterant itself and synthetic sets of adulterated authentic saffron samples. The implementation of the strategy was to measure the amount of an unknown adulterant from the crude extract using curcumin as a non-isotopic isobaric internal standard. The relationship between the saffron and curcumin molar ratios were established with a correlation coefficient of 0.9942. The ANOVA regression model was significant, F(1, 72) = 13595.82, p 0.001, y = (0.0116 +/- 0.0001)x + (-0.1214 +/- 0.0086). No matrix effects were observed and good results were obtained with respect to instrumental repeatability (*RSD% 2%) and LOD (1.1%). The analysis of commercial samples of saffron using the proposed approach showed the suitability of the method for routine analysis (minimal sample preparation and very short measuring time per sample).
机译：这里，我们通过直接MS和MS / MS分析来描述一种快速，简单，可靠的方法，用于藏红花（番红花Sativus L.）的定量分析和分子物种指纹识别。通过实验，用0.3％TFA水/乙腈溶液进行粉状甘油烷烃，将所得混合物直接置于马尔达板上进行分析。这种方法允许检测通常观察到的鳄鱼C-1-C-6和黄酮醇，以及鉴定未知的高糖基化的鳄鱼C-7，C-8和C-9，以及类胡萝卜素衍生的代谢物。该策略认识使用掺假物本身的粗提物和掺杂掺杂的正宗藏红花样品的原油提取物的同时检测和表征藏红花和掺杂标记物。该策略的实施是测量使用姜黄素从原油提取物中的未知掺杂剂的量，作为非同位素同住的内标。用0.9942的相关系数建立甘薯和姜黄素摩尔比之间的关系。 ANOVA回归模型很大，F（1,72）= 13595.82，P＆ 0.001，y =（0.0116 +/- 0.0001）x +（-0.1214 +/- 0.0086）。没有观察到基质效应，并相对于仪器重复性（* RSD％＆LT; 2％）和LOD（1.1％）获得了良好的结果。使用所提出的方法分析藏红花的商业样品展示了常规分析方法的适用性（最小样品制备和每个样品的测量时间非常短）。

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