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A designable aminophenylboronic acid functionalized magnetic Fe3O4/ZIF-8/APBA for specific recognition of glycoproteins and glycopeptides

机译：用于特异性识别糖蛋白和糖肽的可名称氨基苯硼酸官能化磁FE3O4 / ZIF-8 / APBA

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摘要

We fabricated a novel aminophenylboronic acid functionalized magnetic Fe3O4/zeolitic imidazolate framework-8/APBA (denoted as Fe3O4/ZIF-8/APBA). First, Fe3O4 was coated by zeolitic imidazolate framework-8 (denoted as Fe3O4/ZIF-8) using the hydrothermal method. Next, the phenylboronic acid functionalized triethoxysilane reagent was synthesized by 3-aminophenylboronic acid and 3-isocyanatopropyltriethoxysilane, which was modified on the surface of the Fe3O4/ZIF-8 nanocomposite through the sol-gel technique and electrostatic interaction as well as pi-pi stacking interaction. The synthetic Fe3O4/ZIF-8/APBA exhibited high adsorption capacity and good specificity toward glycoproteins. Moreover, the Fe3O4/ZIF-8/APBA possessed high saturation magnetization (51.41 emu g(-1)) and achieved better separation in the presence of an external magnetic field. Above all, the asdesigned Fe3O4/ZIF-8/APBA was successfully used to capture glycoproteins and identify the horseradish peroxidase (HRP) tryptic digest. This study provides a facile strategy to embellish the aminophenylboronic acid onto the nanocomposite substrate and develop a new material for the specific recognition and enrichment of glycoproteins and low-abundance glycopeptides in proteomics research.
机译：我们制造了一种新型氨基苯基硼酸官能化磁FE3O4 /沸石咪唑酯框架-8 / APBA（表示为FE3O4 / ZIF-8 / APBA）。首先，四氧化三铁是通过使用水热法沸石咪唑酯骨架-8（表示为Fe3O4的/ ZIF-8）涂覆。接下来，通过3-氨基苯基硼酸和3-异氰酸丙基丙基三氧基硅烷合成苯基硼酸官能化三乙氧基硅烷试剂，其通过溶胶 - 凝胶技术和静电相互作用以及PI-PI堆叠在Fe3O4 / ZIF-8纳米中复合的表面上进行改性相互作用。合成Fe3O4 / ZIF-8 / APBA表现出高吸附能力和对糖蛋白的良好特异性。此外，Fe3O4 / ZIF-8 / APBA具有高饱和磁化强度（51.41 emug（-1）），并在外部磁场的存在下实现更好的分离。最重要的是，Asdesigned Fe3O4 / ZIF-8 / APBA成功地用于捕获糖蛋白并鉴定辣根过氧化物酶（HRP）胰蛋白酶消化。该研究提供了一种容易策略，将氨基苯基硼酸向纳米复合材料底物装饰在纳米复合材料基质上，并在蛋白质组学研究中制造糖蛋白和低丰度糖肽的特异性识别和富集的新材料。

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  • 来源
    《RSC Advances》 |2018年第13期|共6页
  • 作者

    Li Shanshan; Li Dongyan; Sun Long; Yao Yuewei; Yao Cheng;

  • 作者单位

    Nanjing Tech Univ Sch Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Tech Univ Sch Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Tech Univ Sch Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Tech Univ Sch Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

    Nanjing Tech Univ Sch Chem &

    Mol Engn Nanjing 211816 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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