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The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study

机译：大鼠血浆中伊氏素测定的HPLC-MS / MS方法的开发及验证及其在药代动力学研究中的应用

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摘要

Eriocitrin is one of the major active constituents of lemon fruit, and it possesses strong antioxidant, lipid-lowering, anticancer and anti-inflammatory activities and has long been used in food, beverages and wine. In this study, for the first time, a rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry method (LC/MS/MS) with protein precipitation was developed and validated for the analysis of eriocitrin in rat plasma. Chromatographic separation was achieved using a mobile phase, comprising 0.1% formic acid aqueous solution and acetonitrile eluted at a flow rate of 0.8 mL min(-1). In multiple reaction monitoring (MRM) modes, eriocitrin and internal standard (IS) were quantified using precursor-to-product ion transitions of m/z 595.4 -> 287.1 and m/z 252.0 -> 155.9, respectively. The intra- and inter-day precision (RSD) were below 6.79% in plasma, while accuracy (RE) was within +/- 7.67%. The matrix effect, recovery and stability were also demonstrated to be within acceptable limits. This method was successfully employed in the pharmacokinetic study on rats after the oral administration of eriocitrin. The pharmacokinetic parameters show that the maximum plasma concentration (C-max) of eriocitrin was 299.833 +/- 16.743 mu g L-1, while the corresponding time to reach C-max(T-max) was 0.094 +/- 0.019 h, and the half-time (T-1/2) was 1.752 +/- 0.323 h. The present results would be valuable for further research and development of eriocitrin.
机译：Eriocitrin是柠檬水果的主要活性成分之一，它具有强烈的抗氧化剂，降脂，抗癌和抗炎活动，并长期以来用于食品，饮料和葡萄酒。在本研究中，首次进行快速，选择性和敏感的液相色谱 - 串联质谱法（LC / MS / MS），并验证了大鼠血浆中伊铁蛋白的分析。使用流动相实现色谱分离，其包含0.1％甲酸水溶液和以0.8mL min（-1）的流速洗脱的乙腈。在多反应监测（MRM）模式中，使用M / Z 595.4 - > 287.1和M​​ / Z 252.0-> 155.9的前体 - 产物离子转变来定量二柠檬蛋白和内标（IS）。血浆中的日内和日间精度（RSD）低于6.79％，而精度（重新）在+/- 7.67％内。还证明了基质效应，回收和稳定性是可接受的限制。在伊铁蛋白口服施用后的大鼠药代动力学研究中成功地使用该方法。药代动力学参数表明，伊铁蛋白的最大血浆浓度（C-MAX）为299.833 +/-16.743μgl-1，而相应的达到C-max（t-max）的时间为0.094 +/- 0.019h，半时间（T-1/2）为1.752 +/- 0.323小时。目前的结果对于Eriocitrin的进一步研究和发展是有价值的。

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  • 来源
    《RSC Advances》 |2020年第18期|共7页
  • 作者

    Li Luya; Feng Rui; Feng Xue; Chen Yuting; Liu Xin; Sun Wenjing; Zhang Lantong;

  • 作者单位

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal Shijiazhuang 050017 Hebei Peoples R China;

    Hebei Med Univ Hosp 4 Dept Pharm Shijiazhuang 050011 Hebei Peoples R China;

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal Shijiazhuang 050017 Hebei Peoples R China;

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal Shijiazhuang 050017 Hebei Peoples R China;

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal Shijiazhuang 050017 Hebei Peoples R China;

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal Shijiazhuang 050017 Hebei Peoples R China;

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal Shijiazhuang 050017 Hebei Peoples R China;

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