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首页> 外文期刊>RSC Advances >A novel nanoparticle loaded with methyl caffeate and caffeic acid phenethyl ester against Ralstonia solanacearum-a plant pathogenic bacteria
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A novel nanoparticle loaded with methyl caffeate and caffeic acid phenethyl ester against Ralstonia solanacearum-a plant pathogenic bacteria

机译：用甲基咖啡因和咖啡酸苯乙烷的新型纳米颗粒对抗Ralstonia solanacearum-a植物病原细菌

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摘要

Developing a novel agent and understanding the interaction model between multipolymer nanoparticles and bacteria could be worthwhile to induce the protection of crops with the prevalence of frequent hazards because of the use of pesticides and chemical resistance. Unlike metal nanoparticles, multipolymer nanoparticles have bacteriostatic properties against Ralstonia solanacearum that can trigger bacterial wilt by infecting the plant. Therefore, a novel poly(lactic-co-glycolic acid) nanoparticle containing caffeic acid phenethyl ester (CAPE) and methyl caffeate (MC) was prepared with the sustained-release property (for 10 d at pH 6.5); here, 50% of the cumulative release rate was achieved. It was observed that the cytomembrane of R. solanacearum was jeopardized by the nanoparticle by the creation of large holes on the bacterial surface. The nanoparticle has an approximate EC50 value of 0.285 mg mL(-1) with active pharmaceutical ingredients (APIs), while the drug dosage could be reduced by 2/3. Furthermore, to reveal the possible mechanism of interaction between the multipolymer nanoparticles and bacteria, a formidable inhibition effect was observed; the pathogenicity-related genes, namely, phcA, phcB, pehC, egl, pilT, and polA, of R. solanacearum were downregulated by 1/2, 1/42, 1/13, 1/6, 1/2, and 1/8, respectively, showing significant effects on the major virulence-related genes. Hence, a novel nanoparticle with excellent antibacterial and sustained-release properties has been prepared, possessing the potential to replace chemical pesticides and serve as a new control strategy for mulberry blight disease.
机译：开发一种新型药剂并理解多聚体纳米粒子和细菌之间的相互作用模型可能是值得诱导农作物的保护，因为使用杀虫剂和耐化学性而常见的危害患病率。与金属纳米颗粒不同，多聚糖纳米颗粒具有对Ralstonia Solanacearum的抑菌性能，可以通过感染植物来引发细菌枯萎病。因此，用缓释的性质（pH6.5在pH6.5处为10d）制备含有咖啡酸苯乙烷酯（己酸苯乙烷酯（己酰）和甲基咖啡因（MC）的新型聚（乳酸二乙醇酸）纳米颗粒;这里，实现了50％的累积释放率。观察到R.Solanacearum的细胞膜通过在细菌表面上产生大孔而受到纳米颗粒的危害。纳米颗粒具有0.285mg ml（-1）的近似EC50值，具有活性药物成分（API），而药物剂量可降低2/3。此外，为了揭示多聚物纳米颗粒和细菌之间的相互作用机制，观察到突起的抑制效果;将致病性相关的基因，即R.Solanacearum的PHCA，PHCB，PEHC，EGL，PILT和POLA，下调1/2,1 / 42,113,11 / 6,1 / 2和1 / 8分别显示出对主要毒力相关基因的显着影响。因此，已经制备了具有优异的抗菌和缓释性能的新型纳米颗粒，具有替代化学杀虫剂的潜力，并作为桑椹枯萎病的新控制策略。

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  • 来源
    《RSC Advances》 |2020年第7期|共13页
  • 作者

    Wang Jin-Zheng; Yan Cheng-Hai; Zhang Xiao-Rui; Tu Qing-Bo; Xu Yan; Sheng Sheng; Wu Fu-An; Wang Jun;

  • 作者单位

    Jiangsu Univ Sci &

    Technol Sch Biotechnol Zhenjiang 212018 Jiangsu Peoples R China;

    Jiangsu Univ Sci &

    Technol Sch Biotechnol Zhenjiang 212018 Jiangsu Peoples R China;

    Jiangsu Univ Sci &

    Technol Sch Biotechnol Zhenjiang 212018 Jiangsu Peoples R China;

    Jiangsu Univ Sci &

    Technol Sch Biotechnol Zhenjiang 212018 Jiangsu Peoples R China;

    Jiangsu Univ Sci &

    Technol Sch Biotechnol Zhenjiang 212018 Jiangsu Peoples R China;

    Jiangsu Univ Sci &

    Technol Sch Biotechnol Zhenjiang 212018 Jiangsu Peoples R China;

    Jiangsu Univ Sci &

    Technol Sch Biotechnol Zhenjiang 212018 Jiangsu Peoples R China;

    Jiangsu Univ Sci &

    Technol Sch Biotechnol Zhenjiang 212018 Jiangsu Peoples R China;

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