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首页> 外文期刊>RSC Advances >Novel bovine carbonic anhydrase encapsulated in a metal-organic framework: a new platform for biomimetic sequestration of CO2
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Novel bovine carbonic anhydrase encapsulated in a metal-organic framework: a new platform for biomimetic sequestration of CO2

机译：包装在金属有机框架中的新型牛碳酸酐基：CO2的仿生仿真的新平台

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In this work, maximizing the utilization of CO2 and its precipitation as CaCO3 by using immobilized bovine carbonic anhydrase (BCA) was evaluated. In this way, selection of suitable carriers which have a gas adsorption function would enhance the CO2 sequestration efficiency of the carbonic anhydrase (CA). So a metal-organic framework (MOF), an excellent material for gas adsorption and enzyme immobilization was used. In this manner, BCA was encapsulated into the microporous zeolite imidazolate framework, ZIF-8, for the first time, using a bottle-around-a-ship method. Systematic characterization including powder X-ray diffraction (PXRD), UV-vis, and Fourier transform infrared (FT-IR) spectroscopies, BET, field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray (EDX) confirmed that the entrapment of BCA molecules was successfully achieved during the crystal growth of ZIF-8 with an enzyme loading of ca. 100 +/- 1.2 mg g(-1) of BCA-ZIF-8. Optimization of the matrix for increasing the stability of the enzyme in an encapsulated form is the main aim of the present study. The de novo approach was proposed because this method provides better enzyme protection from degradation, minimizes enzyme leaching and enables multiple reuse. Then, the influence of different parameters, including pH, temperature, storage and reusability, was evaluated for enzyme@MOF composites versus free enzymes. The prepared biocatalyst exhibited outstanding activity in a wide pH and temperature range and demonstrates high storage stability up to 37 days. This efficient and simple association procedure seems well-adapted to produce an enzymatic bio-catalyst for biocatalytic hydration of CO2. The FT-IR analysis revealed that the structure of BCA was well maintained during the encapsulation process. The thermal stability and reusability of the BCA-ZIF-8 increased noticeably due to the structural rigidity and confinement of the ZIF-8 scaffolds. These two parameters are very important for practical applications.
机译：在这项工作中，通过使用固定化的牛碳酸酐酶（BCA）最大化CO 2的利用率和它的以CaCO 3沉淀进行评价。以这种方式，其具有气体吸附功能将增强碳酸酐酶（CA）的CO 2隔离效率合适的载体的选择。所以，使用了金属 - 有机构架（MOF），用于气体吸附和酶固定化的优良材料。以这种方式，BCA被封装到微孔沸石咪唑酯骨架结构，ZIF-8，对于第一次，使用的瓶周围-A-船方法。系统的特征包括粉末X射线衍射（PXRD），UV-VIS，和傅里叶变换红外（FT-IR）光谱，BET，场发射扫描电子显微镜（FE-SEM）和能量色散X射线（EDX）证实BCA分子的包封ZIF-8的用约的酶用量在晶体生长期间，成功地实现了100 +/- 1.2毫克克（-1）BCA-ZIF-8的。矩阵用于增加包封形式的酶的稳定性的优化是本研究的主要目的。提出了从头的方法，因为这种方法提供了免于降解，最小化酶浸出更好酶保护和能够使多个再利用。然后，不同的参数，包括pH值，温度，储存和可重用性的影响，进行了评估酶@ MOF复合材料相对于游离酶。将制得的生物催化剂在宽的pH和温度范围内表现出显着活性，并演示高储存稳定性可达37天。这种有效且简单的关联过程似乎很好地适应，以产生二氧化碳的生物催化水合酶的生物催化剂。的FT-IR分析表明，BCA的结构在封装工艺期间被保持良好。的BCA-ZIF-8的热稳定性和可重用性显着的增加，由于ZIF-8的支架的结构刚性和禁闭。这两个参数在实际应用中非常重要。

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    《RSC Advances》 |2019年第49期|共10页
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