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首页> 外文期刊>RSC Advances >LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b
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LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b

机译：LNCRNA Hotism1通过靶向miR-148b参与急性髓细胞白血病的进展

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摘要

LncRNAs have been shown to be involved in the biological and pathological processes of acute myeloid leukemia (AML). Hox antisense intergenic RNA myeloid 1 (HOTAIRM1) was reported to be highly expressed in AML. However, the detailed role and molecular mechanism of HOTAIRM1 in AML pathogenesis remain undefined. In the present study, HOTAIRM1 and miR-148b expressions in AML patients and healthy controls were detected by qRT-PCR. Cell proliferation and apoptosis were evaluated by CCK-8 and flow cytometry assays, respectively. The regulatory interaction between HOTAIRM1 and miR-148b was explored by bioinformatics analysis using starBase v3.0 software and The Cancer Genome Atlas (TCGA) AML dataset. We found that the miR-148/miR-152 family members including miR-148a, miR-148b, and miR-152 were predicted to be potential targets of HOTAIRM1. HOTAIRM1 expression was negatively correlated with miR-148b expression but had no correlation with miR-148a/miR-152 expressions in AML samples from the TCGA dataset. HOTAIRM1 expression was higher while miR-148b expression was lower in AML patients than in healthy controls. A negative correlation between HOTAIRM1 and miR-148b in AML patients was observed. HOTAIRM1 silencing and miR-148b overexpression both suppressed cell proliferation and induced apoptosis in AML cells. miR-148b was identified as a target of HOTAIRM1 in AML cells. Moreover, HOTAIRM1 knockdown inhibited proliferation and induced apoptosis in AML cells by negatively regulating miR-148b. In summary, HOTAIRM1 was involved in the progression of AML through targeting miR-148b, shedding light on the biological function and molecular mechanism of HOTAIRM1 in AML.
机译：已显示LNCRNA参与急性髓性白血病（AML）的生物学和病理过程。据报道，霍克斯反义亚核酸RNA骨髓1（Hotism1）在AML中高度表达。然而，AML发病机制中Hotism1的详细作用和分子机制仍然是未定义的。在本研究中，通过QRT-PCR检测AML患者和健康对照中的Hotism1和MiR-148b表达。通过CCK-8和流式细胞术测定评估细胞增殖和细胞凋亡。使用Starbase V3.0软件和癌症基因组图集（TCGA）AML数据集，通过生物信息化分析探索了Hotism1和MiR-148b之间的调节相互作用。我们发现，预计包括MIR-148A，MIR-148B和MIR-152的MIR-148 / MIR-152家庭成员是Hotism1的潜在目标。 Hotism1表达与miR-148b表达呈负相关，但与来自TCGA数据集的AML样本中的MIR-148A / miR-152表达无关。 Hotism1表达较高，而MiR-148b表达在AML患者中较低，而不是健康对照。观察到AML患者HotaIrm1和miR-148b之间的负相关性。 Hotism1沉默和miR-148b过表达抑制细胞增殖和诱导AML细胞的细胞凋亡。 MiR-148B被鉴定为AML细胞中HotaIrm1的靶标。此外，Hotism1通过负调节miR-148b抑制AML细胞中的增殖和诱导细胞凋亡。总之，HotisM1通过靶向MIR-148B参与AML的进展，脱落在AML中Hotism1的生物学功能和分子机制。

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  • 来源
    《RSC Advances》 |2019年第18期|共8页
  • 作者

    Hu Ning; Chen Li; Li Qianyu; Zhao Hongmian;

  • 作者单位

    Henan Univ Dept Hematol Huaihe Hosp 115 Ximen St Kaifeng 475000 Henan Peoples R China;

    Henan Univ Dept Hematol Huaihe Hosp 115 Ximen St Kaifeng 475000 Henan Peoples R China;

    Henan Univ Dept Hematol Huaihe Hosp 115 Ximen St Kaifeng 475000 Henan Peoples R China;

    Henan Univ Dept Hematol Huaihe Hosp 115 Ximen St Kaifeng 475000 Henan Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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