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首页> 外文期刊>RSC Advances >An innovative biotransformation to produce resveratrol by Bacillus safensis
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An innovative biotransformation to produce resveratrol by Bacillus safensis

机译：通过芽孢杆菌生物生物转化制作生物转化，由Bacillus Safensis生产白藜芦醇

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摘要

Resveratrol is considered as a potential food supplement, cosmetic ingredient and nutraceutical. In this study, resveratrol was produced by biotransformation successfully. In detail, a -glucosidase producing strain was isolated and identified as Bacillus safensis, and it could convert polydatin to resveratrol efficiently and rapidly. Further research showed that the conversion rate to resveratrol reached 93.1% in 8 h at 37 degrees C. The production of resveratrol was confirmed by HPLC, LC-MS and H-1-NMR to identify its structure and it was verified to possess antibacterial properties especially against Escherchia coli. To illustrate the resveratrol transformation mechanism, several glucosidases from B. safensis CGMCC 13129 were expressed and analyzed. The results showed that BGL4 and BGL5 had higher transformation activity compared with other tested glucosidases. This research provides a novel approach to produce resveratrol, and would promote the application of resveratrol in health-promoting pharmaceutical and food products.
机译：白藜芦醇被认为是潜在的食品补充剂，化妆品成分和营养保健品。在这项研究中，白藜芦醇成功通过生物转化产生。详细地，分离出戊糖苷酶产生菌株并将其鉴定为芽孢杆菌安全性，并且可以将多达丁转化为有效且迅速地将多角子转化为白藜芦醇。进一步的研究表明，在37摄氏度下将转化率达到93.1％达到93.1％。通过HPLC，LC-MS和H-1-NMR证实了白藜芦醇以鉴定其结构，验证其具有抗菌性质特别是反对大肠杆菌。为了说明白藜芦醇转化机制，表达并分析了来自B. Safensis CGMCC 13129的几种葡糖苷酶。结果表明，与其他测试的葡糖苷酶相比，BGL4和BGL5具有更高的转化活性。本研究提供了一种生产白藜芦醇的新方法，并促进白藜芦醇在促进促进药品和食品的应用。

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  • 来源
    《RSC Advances》 |2019年第27期|共9页
  • 作者

    Hu Xiaoyan; Liu Yexue; Li Dengke; Feng Wei; Ni Hanmeng; Cao Shan; Lu Fuping; Li Yu;

  • 作者单位

    Tianjin Univ Sci &

    Technol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Qilu Univ Technol Coll Leather Chem &

    Engn Jinan Shandong Peoples R China;

    Tianjin Univ Sci &

    Technol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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