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首页> 外文期刊>RSC Advances >Anti-inflammatory effects of paeoniflorin from Paeonia lactiflora Pall. on human corneal epithelial cells and a mouse model of dry eye disease
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Anti-inflammatory effects of paeoniflorin from Paeonia lactiflora Pall. on human corneal epithelial cells and a mouse model of dry eye disease

机译：Paeonia Lactiflora Pall芍药苷的抗炎作用。 在人角膜上皮细胞和干眼症小鼠模型

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摘要

Dry eye disease (DED) is characterized by increased osmolality of tears due to a lack of production or increased evaporation of tears. Hyperosmolarity is involved in DED pathogenesis, which damages ocular surface cells and leads to inflammation of the ocular surface. We investigated the anti-inflammatory effect of paeoniflorin (PF) from Paeonia lactiflora Pall. on human corneal epithelial (HCE) cells and its molecular mechanisms, and its therapeutic effects on a mouse model of experimental dry eye (EDE). HCE cells were treated with PF-1 (PF prepared in vitro; 0.01%, 0.1% and 1.0%). Protein production/activity was determined by Western blotting, RT-PCR and immunofluorescent staining. Meanwhile, eye drops containing 0.01%, 0.1% and 1.0% of PF-2 (PF prepared in vivo) were applied to the EDE, and the tear volume, corneal fluorescein-staining score, detachment of the corneal epithelium, and immunohistochemical staining were measured after 28 days of treatment. PF reduced expression of proinflammatory factors such as interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF)- in HCE cells, and significantly improved dry-eye signs, including tear volume, desquamation of the corneal epithelium and ocular surface inflammation in mice treated with 1.0% PF-2. Further study showed that PF improved EDE by inhibiting mitogen-activated protein kinase (MAPK), phosphorylated (p)-c-Jun N-terminal kinase (JNK) and pp-38, and nuclear factor kappa B (NF-B) signaling pathways. These data suggest that PF can improve dry-eye symptoms and reduce expression of proinflammatory mediators. Hence, eye drops containing PF could be used as an adjunctive treatment for DED.
机译：由于缺乏生产或泪液蒸发而增加，干眼症（DED）的特征是渗透性增加。 Hygermolarity参与了DED发病机制，其损坏了眼部表面细胞并导致眼表面的炎症。我们研究了Paeonia Lactiflora Pall的Paeoniflorin（PF）的抗炎作用。对人角膜上皮（HCE）细胞及其分子机制，及其对实验性干眼（EDE）小鼠模型的治疗效果。用PF-1处理HCE细胞（体外制备的PF; 0.01％，0.1％和1.0％）。通过蛋白质印迹，RT-PCR和免疫荧光染色测定蛋白质产生/活性。同时，将含有0.01％，0.1％和1.0％PF-2（体内PF的PF）的眼滴，并施加到eDE中，撕裂体积，角膜荧光素染色得分，角膜上皮脱落和免疫组化染色治疗28天后测量。 PF降低的促炎因子的表达，如白介素（IL）-1，IL-6和肿瘤坏死因子（TNF） - 在HCE细胞和显著改善干眼症体征，包括泪液量，角膜上皮和眼的脱屑用1.0％PF-2处理的小鼠中表面炎症。进一步的研究表明，通过抑制丝裂原激活的蛋白激酶（MAPK），磷酸化（P）-C-JUN N-末端激酶（JNK）和PP-38，以及核因子Kappa B（NF-B）信号通路的PF改善了EDE 。这些数据表明，PF可以改善干眼症症状并减少促炎介质的表达。因此，含有PF的眼药水可用作DED的辅助治疗。

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  • 来源
    《RSC Advances》 |2019年第23期|共9页
  • 作者

    Zhao Mincong; Liu Li; Zheng Yating; Liu Guangrong; Che Biao; Li Penghui; Chen Huixiong; Dong Changzhi; Lin Li; Du Zhiyun;

  • 作者单位

    Guandong Univ Technol Sch Biomed &

    Pharmaceut Engn Inst Nat Med &

    Green Chem Guangzhou 510006 Guangdong Peoples R China;

    Infinitus China Co Ltd Jiangmen 529156 Peoples R China;

    Guandong Univ Technol Sch Biomed &

    Pharmaceut Engn Inst Nat Med &

    Green Chem Guangzhou 510006 Guangdong Peoples R China;

    Infinitus China Co Ltd Jiangmen 529156 Peoples R China;

    Infinitus China Co Ltd Jiangmen 529156 Peoples R China;

    Guandong Univ Technol Sch Biomed &

    Pharmaceut Engn Inst Nat Med &

    Green Chem Guangzhou 510006 Guangdong Peoples R China;

    Guandong Univ Technol Sch Biomed &

    Pharmaceut Engn Inst Nat Med &

    Green Chem Guangzhou 510006 Guangdong Peoples R China;

    Guandong Univ Technol Sch Biomed &

    Pharmaceut Engn Inst Nat Med &

    Green Chem Guangzhou 510006 Guangdong Peoples R China;

    Allan Conney Biotechnol Co Ltd Foshan 528000 Peoples R China;

    Guandong Univ Technol Sch Biomed &

    Pharmaceut Engn Inst Nat Med &

    Green Chem Guangzhou 510006 Guangdong Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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