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首页> 外文期刊>RSC Advances >Characterization and molecular docking of new 17 fatty acid desaturase genes from Rhizophagus irregularis and Octopus bimaculoides
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Characterization and molecular docking of new 17 fatty acid desaturase genes from Rhizophagus irregularis and Octopus bimaculoides

机译：根瘤菌和八达通骨素生物发射术中新型17脂肪酸去饱和酶基因的特征及分子对接

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摘要

Fatty acid desaturases are key enzymes in the biosynthesis of n-3 polyunsaturated fatty acids (PUFAs) via conversion of n-6 polyunsaturates to their n-3 counterparts. In this study, we reported the characterization and molecular docking of 17 desaturases from Rhizophagus irregularis and Octopus bimaculoides. These two new desaturase genes were screened using the known 17 desaturase gene (oPaFADS17) from Pythium aphanidermatum as a template. Analysis of their genes revealed that the sequences of oRiFADS17 and oObFADS17 contained the typical His-rich motifs (one HXXXH and two HXXHH). They were then expressed in Saccharomyces cerevisiae INVSc1 to examine their activities and substrate preferences. Our results show that the two candidate n-3 desaturases possess a strong 17 desaturase activity, exhibiting remarkable increase in desaturation activity on C20 fatty acids compared to C18 fatty acids. To the best of our knowledge, oRiFADS17 desaturase has greater (3-4 fold) catalytic activity for C18 substrates than other reported 17 desaturases and oObFADS17 is the first reported 17 desaturase in sea mollusks. Characterization of these two new desaturases will be of greater value for genetic engineering in industrial production of eicosapentaenoic acid (EPA, C20:5n-3) and docosahexaenoic acid (DHA, C22:6n-3). Due to lack of crystal structure information about n-3 desaturases, for the first time, the view of their predicted structures, binding pockets and substrate tunnels was clearly observed based on molecular docking. This will contribute to strengthening our understanding of the structure-function relationships of n-3 fatty acid desaturases.
机译：脂肪酸去饱和酶是N-3多不饱和脂肪酸（PUFAS）的密钥酶，通过将N-6多不饱和酸盐转化为它们的N-3对应物。在这项研究中，我们报道了来自根鼻葵和八达通血管素17个去饱和酶的表征和分子对接。使用来自富硫酸戊酰胺属的已知的17个去饱和酶基因（OPAFADS17）作为模板，筛选这两个新的去饱和酶基因。对其基因的分析显示，奥里芬人17和Oobfads17的序列含有典型的他的富含族的基序（一种HXXXH和两种HXXHH）。然后，它们在酿酒酵母中表达酿酒酵母INVSC1以检查其活性和基材偏好。我们的结果表明，与C18脂肪酸相比，两种候选N-3去饱和酶具有强大的17个去饱和酶活性，在C20脂肪酸上表现出显着的去饱和活性。据我们所知，ORIFADS17去饱和酶比其他报道的17个去饱和酶和OOBFADS17具有更大的（3-4倍）的催化活性，并且OOBFADS17是海洋软体动物中首先报道的17个去饱和酶。对这两种新的去饱和酶的表征对于产业生产的产业生产（EPA，C20：5N-3）和二十二碳六烯酸（DHA，C22：6N-3）的基因工程将具有更大的价值。由于缺乏关于N-3去饱和酶的晶体结构信息，首次基于分子对接清楚地观察到其预测结构，粘合袋和衬底隧道的视图。这将有助于加强我们对N-3脂肪酸去饱和酶的结构功能关系的理解。

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  • 来源
    《RSC Advances》 |2019年第12期|共10页
  • 作者

    Rong Chunchi; Chen Haiqin; Tang Xin; Gu Zhennan; Zhao Jianxin; Zhang Hao; Chen Wei; Chen Yong Q.;

  • 作者单位

    Jiangnan Univ State Key Lab Food Sci &

    Technol Wuxi Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci &

    Technol Wuxi Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci &

    Technol Wuxi Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci &

    Technol Wuxi Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci &

    Technol Wuxi Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci &

    Technol Wuxi Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci &

    Technol Wuxi Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci &

    Technol Wuxi Jiangsu Peoples R China;

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