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首页> 外文期刊>RSC Advances >A highly selective ratiometric fluorescent probe for the cascade detection of Zn2+ and H2PO4- and its application in living cell imaging
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A highly selective ratiometric fluorescent probe for the cascade detection of Zn2+ and H2PO4- and its application in living cell imaging

机译：一种高精度的比例荧光探针，用于Zn2 +和H2PO4的级联检测及其在活细胞成像中的应用

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摘要

A simple ratiometric sensor (L-1) for the cascade detection of Zn2+ and H2PO4- with high selectivity was reported based on the intermolecular charge transfer (ICT) mechanism. This new sensor could distinguish Zn2+ from Cd2+, and features high fluorescence quantum yield with L-1 (0.45) and L-1-Zn2+ (0.27). There is a good linear relationship between the fluorescence ratio I-525 nm/I-430 nm upon addition of Zn2+ and the limit of detection (LOD) was evaluated to be 41.0 nM. Furthermore, the in situ prepared L-1-Zn2+ complex also displayed good selectivity and ratiometric response for H2PO4- and the detection limit of L-1-Zn2+ was found to be 49.0 nM. L-1 exhibits permeability and can been applied to the detection of intracellular Zn2+ and H2PO4- in HepG-2 cells.
机译：基于分子间电荷转移（ICT）机制，报道了用于Zn2 +和H2PO4的级联检测的简单比率传感器（L-1）。 该新传感器可以将Zn2 +与CD2 +区分开，具有L-1（0.45）和L-1-Zn2 +（0.27）的高荧光量子产率。 在加入Zn2 +后，荧光比I-525nm / I-430nm之间存在良好的线性关系，并评估检测极限（LOD）为41.0nm。 此外，原位制备的L-1-Zn2 +复合物也显示出H2PO 4的良好选择性和比例响应，并且发现L-1-Zn2 +的检测限为49.0nm。 L-1表现出渗透性，可应用于细胞内Zn2 +和H2PO4-在HepG-2细胞中的检测。

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  • 来源
    《RSC Advances》 |2017年第64期|共6页
  • 作者

    Du Kui; Niu Shizhen; Qiao Li; Dou Yandong; Zhu Qing; Chen Xinzhi; Zhang Pengfei;

  • 作者单位

    Zhejiang Univ Coll Chem &

    Biol Engn Minist Educ Key Lab Biomass Chem Engn Hangzhou 310027 Peoples R China;

    Zhejiang Univ Coll Chem &

    Biol Engn Minist Educ Key Lab Biomass Chem Engn Hangzhou 310027 Peoples R China;

    Hangzhou Normal Univ Coll Mat Chem &

    Chem Engn Hangzhou 310036 Zhejiang Peoples R China;

    Coll Biotechnol &

    Bioengn Key Lab Bioorgan Synth Zhejiang Prov Hangzhou 310014 Zhejiang Peoples R China;

    Coll Biotechnol &

    Bioengn Key Lab Bioorgan Synth Zhejiang Prov Hangzhou 310014 Zhejiang Peoples R China;

    Zhejiang Univ Coll Chem &

    Biol Engn Minist Educ Key Lab Biomass Chem Engn Hangzhou 310027 Peoples R China;

    Hangzhou Normal Univ Coll Mat Chem &

    Chem Engn Hangzhou 310036 Zhejiang Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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