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首页> 外文期刊>RSC Advances >Utilization of chromic polydiacetylene assemblies as a platform to probe specific binding between drug and RNA
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Utilization of chromic polydiacetylene assemblies as a platform to probe specific binding between drug and RNA

机译：用铬多迪亚乙烯组件的利用作为探测药物和RNA之间特异性结合的平台

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摘要

Recognition of nucleic acids remains an important endeavor in biology. Nucleic acids adopt shapes ranging from A-form (RNA and GC rich DNA) to B-form (AT rich DNA). We show, in this contribution, shapespecific recognition of A-U rich RNA duplex by a neomycin (Neo)-polydiacetylene (PDA) complex. PDA assemblies are fabricated by using a well-known diacetylene (DA) monomer, 10,12-pentacosadiynoic acid (PCDA). The response of poly(PCDA) assemblies is generated by mixing with a modified neomycin-PCDA monomer (Neo-PCDA). The functionalization by neomycin moiety provides specific binding with homopolyribonucleotide poly(rA)-poly(rU) stimulus. Various types of alcohols are utilized as additives to enhance the sensitivity of poly(PCDA)/Neo-PCDA assemblies. A change of absorption spectra is clearly observed when a relatively low concentration of poly(rA)-poly(rU) is added into the system. Furthermore, poly(PCDA)/Neo-PCDA shows a clear specificity for poly(rA)-poly(rU) over the corresponding DNA duplex. The variation of linker between neomycin moiety and conjugated PDA backbone is found to significantly affect its sensitivity. We also investigate other parameters including the concentration of Neo-PCDA and the DA monomer structure. Our results provide here preliminary data for an alternative approach to improve the sensitivity of PDA utilized in biosensing and diagnostic applications.
机译：核酸的识别仍然是生物学中的重要努力。核酸采用从A形（RNA和GC富含DNA）到B形（富含DNA）的形状。我们展示了在这种贡献中，通过新霉素（Neo）-polydiacetylene（PDA）复合物对A-U富RNA双链体的形状识别。通过使用众所周知的二乙炔（DA）单体，10,12-五酰基甲酸（PCDA）来制造PDA组件。通过与改性的新霉素-CCDA单体（Neo-PCDA）混合来产生聚（PCDA）组件的响应。 NeoMycin部分的官能化提供与均多核苷酸聚（Ra）-poly（Ru）刺激的特异性结合。各种类型的醇类用作添加剂以增强聚（PCDA）/ Neo-PCDA组件的敏感性。当在系统中加入相对较低的聚（Ra）-poly（Ru）时，清楚地观察到吸收光谱的变化。此外，聚（PCDA）/ Neo-PCDA显示在相应的DNA双链体上的聚（Ra）-poly（Ru）的透明特异性。发现新霉素部分和缀合的PDA主链之间的接头的变化显着影响其敏感性。我们还研究了其他参数，包括Neo-PCDA和DA单体结构的浓度。我们的结果提供了替代方法，以提高生物传感和诊断应用中PDA的敏感性的替代方法。

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  • 来源
    《RSC Advances》 |2017年第66期|共9页
  • 作者

    Kamphan Anothai; Gong Changjun; Maiti Krishnagopal; Sur Souvik; Traiphol Rakchart; Arya Dev P.;

  • 作者单位

    Naresuan Univ Fac Sci Dept Chem Phitsanulok 65000 Thailand;

    Clemson Univ Dept Chem Lab Med Chem Clemson SC 29634 USA;

    Clemson Univ Dept Chem Lab Med Chem Clemson SC 29634 USA;

    Clemson Univ Dept Chem Lab Med Chem Clemson SC 29634 USA;

    Naresuan Univ Fac Sci Dept Chem Phitsanulok 65000 Thailand;

    Clemson Univ Dept Chem Lab Med Chem Clemson SC 29634 USA;

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  • 正文语种 eng
  • 中图分类 化学;
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