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首页> 外文期刊>RSC Advances >Combining paired analytical metabolomics and common garden trial to study the metabolism and gene variation of Ginkgo biloba L. cultivated varieties
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Combining paired analytical metabolomics and common garden trial to study the metabolism and gene variation of Ginkgo biloba L. cultivated varieties

机译：结合配对的分析代谢组合和共同的园林试验研究银杏培养品种的新陈代谢和基因变异

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摘要

Secondary metabolites play a pivotal role in plant physiology andmedicinal function. Muchwork has been carried out to uncover the genetic basis of plant secondary metabolite variation, but direct screening of gene variation in the whole genome is extremely time-and labor-consuming. The prediction of a candidate locus of a single nucleotide polymorphism (SNP) will save much time and resource. In this work, we combined a paired analytical metabolomics and a common garden trial to bridge the association of plant metabolism and related gene variation of Ginkgo cultivated varieties. Firstly, the leaves of 30 cultivated varieties of Ginkgo biloba L. grown in the same garden since 1990 were analyzed by UHPLC-QQQ MS/MS. Thirty-six metabolites in the flavonoid biosynthetic pathway were quantified. The biosynthetic rate of flavonoids in different cultivated varieties could reflect the related enzyme gene variation, since the environmental influence was minimized. Thus, the role of SNPs in possible varied genes was further associated with flavonoid synthesis. Results showed that after long term environment and artificial influence, different accessions of G. biloba showed a varied ability in flavonoid aglycone synthesis due to some gene polymorphism; this difference may be heritable but not obvious. Compared with previous methods, this strategy is advantageous in accuracy, low sample requirement and easier operation, providing effective information in phenotype-genotype association, and it can also be used in the heritability study of artificial breeding before large-area introduction.
机译：次生代谢物在植物生理学和相关功能中发挥枢轴作用。已经进行了大量的揭示植物次级代谢物变异的遗传基础，但直接筛查整个基因组的基因变异是极其时间和劳动。预测单个核苷酸多态性（SNP）的候选基因座将节省大量时间和资源。在这项工作中，我们将配对的分析代谢组和共同的园林审判组合在一起，以弥合植物代谢和银杏栽培品种的相关基因变异的关联。首先，通过UHPLC-QQQ MS / MS分析了自1990年以来在同一庭院中生长的30种栽培品种的银杏叶。量化了黄酮类生物合成途径中的三十六种代谢物。不同栽培品种中黄酮类化合物的生物合成率可以反映相关酶基因变异，因为对环境影响最小化。因此，SNP在可能的变化基因中的作用进一步与黄酮合成相关。结果表明，在长期环境和人工影响之后，由于一些基因多态性，G.Biloba的不同含量显示出的黄酮糖苷糖苷合成。这种差异可能是遗传但不明显的。与先前的方法相比，该策略的精度是有利的，精度低，样品要求和更容易操作，提供了表型基因型协会中的有效信息，并且还可以用于大面积介绍之前人工育种的可遗传性研究。

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  • 来源
    《RSC Advances》 |2017年第87期|共9页
  • 作者

    Liu Xin-Guang; Lu Xu; Wang Ji-Xin; Wu Bin; Lin Lin; Wang Hui-Ying; Guo Ru-Zhou; Li Ping; Yang Hua;

  • 作者单位

    China Pharmaceut Univ State Key Lab Nat Med 24 Tongjia Lane Nanjing 210009 Jiangsu Peoples R China;

    China Pharmaceut Univ State Key Lab Nat Med 24 Tongjia Lane Nanjing 210009 Jiangsu Peoples R China;

    China Pharmaceut Univ State Key Lab Nat Med 24 Tongjia Lane Nanjing 210009 Jiangsu Peoples R China;

    China Pharmaceut Univ State Key Lab Nat Med 24 Tongjia Lane Nanjing 210009 Jiangsu Peoples R China;

    China Pharmaceut Univ State Key Lab Nat Med 24 Tongjia Lane Nanjing 210009 Jiangsu Peoples R China;

    China Pharmaceut Univ State Key Lab Nat Med 24 Tongjia Lane Nanjing 210009 Jiangsu Peoples R China;

    China Pharmaceut Univ State Key Lab Nat Med 24 Tongjia Lane Nanjing 210009 Jiangsu Peoples R China;

    China Pharmaceut Univ State Key Lab Nat Med 24 Tongjia Lane Nanjing 210009 Jiangsu Peoples R China;

    China Pharmaceut Univ State Key Lab Nat Med 24 Tongjia Lane Nanjing 210009 Jiangsu Peoples R China;

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