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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Heterogeneity of reticulocyte population in mouse peripheral blood
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Heterogeneity of reticulocyte population in mouse peripheral blood

机译：小鼠外周血网织红细胞群的异质性

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Reticulocytes constitute about 1-6% of total blood erythrocyte in mice and their numbers may be upregulated markedly in a variety of situations like treatment with erythropoietin and induction of anaemia, etc. Reticulocytes originate in the bone marrow from erythroblasts by the process of nuclear extrusion, and are released into the blood where they further mature into erythrocytes. From blood cells, reticulocytes may be isolated using discontinuous Percoll density gradient (DPDG) fractionation. Highly enriched reticulocyte preparations are obtained from the low buoyant density cellular fraction from DPDG and such enriched cell preparations have as such been used extensively as a source of purified blood reticulocytes in many studies. The possibility of presence of reticulocytes in other cell fractions of higher buoyant densities has, however, not been examined. In the present study, we have fractionated mouse blood cells on a five-layered discontinuous DPDG and the presence of reticulocytes was monitored in each fraction by staining for Ter-119 (transferrin receptor) and CD71, markers that together are specific markers for blood-derived reticulocytes. Our results indicate that only 16% of the blood reticulocytes" were present in the low buoyant density DPDG fraction, the rest being distributed in heavier DPDG fractions. Expression levels of some important functional and phenotypic reticulocyte markers like CD47 (integrin associated protein), CD147 (basigin), cellular calcium levels as well as RNA contents were compared for reticulocyte populations derived from different DPDG fractions. Our results show significant differences in the expression of these markers in reticulocyte populations of different buoyant densities and indicate that the reticulocytes isolated from low buoyant density fractions of blood cells may represent only a minor subpopulation of blood reticulocytes.
机译：网织红细胞约占小鼠总血红细胞的1-6％，在各种情况下（如用促红细胞生成素治疗和诱发贫血等），其数量可能显着上调。网织红细胞通过核挤压过程从成红细胞起源于骨髓并释放到血液中，然后它们进一步成熟为红细胞。可以使用不连续的Percoll密度梯度（DPDG）分级分离法从血细胞中分离网状细胞。从DPDG的低浮力细胞级分获得高富集的网状红细胞制品，并且这种富集的细胞制品在许多研究中已被广泛用作纯化的血液网状红细胞的来源。然而，尚未检查网状细胞是否在其他具有较高浮力密度的细胞部分中存在的可能性。在本研究中，我们在五层不连续的DPDG上分离了小鼠血细胞，并通过对Ter-119（转铁蛋白受体）和CD71进行染色来监测每个部分中网状细胞的存在，这些标记一起是血液的特异性标记，衍生的网状细胞。我们的结果表明，低浮力密度DPDG组分中仅存在16％的血液网织细胞，其余分布在较重的DPDG组分中。一些重要的功能性和表型网织细胞标志物的表达水平，例如CD47（整合素相关蛋白），CD147 （basigin），比较了来自不同DPDG组分的网织红细胞群体的细胞钙水平和RNA含量，我们的结果表明，这些标志物在不同浮力密度的网织红细胞群体中的表达存在显着差异，并表明从低浮力中分离出的网织红细胞血细胞的密度分数可能仅代表血液网织红细胞的次要亚群。

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